Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/- |
| Location | 407..932 | Replicon | plasmid pRSB225 |
| Accession | NC_025100 | ||
| Organism | uncultured bacterium | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | - |
| Locus tag | pRSB225_2 | Protein ID | YP_009065961.1 |
| Coordinates | 627..932 (+) | Length | 102 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | - |
| Locus tag | pRSB225_1 | Protein ID | YP_009065960.1 |
| Coordinates | 407..625 (+) | Length | 73 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| pRSB225_1 | 407..625 | + | 219 | YP_009065960.1 | Post-segregation antitoxin CcdA (ccd killing mechanism protein) | Antitoxin |
| pRSB225_2 | 627..932 | + | 306 | YP_009065961.1 | plasmid maintenance protein toxin component | Toxin |
| pRSB225_3 | 933..1739 | + | 807 | YP_009065962.1 | resolvase ResD | - |
| pRSB225_4 | 2624..3268 | + | 645 | YP_009065963.1 | Replication initiation protein RepE | - |
| pRSB225_5 | 3856..5022 | + | 1167 | YP_009065964.1 | plasmid partition protein SopA | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | aph(3')-Ia / aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / mph(A) / dfrA14 / catA1 / tet(B) / sitABCD | iutA / iucD / iucC / iucB / iucA | 1..164550 | 164550 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 102 a.a. Molecular weight: 11706.51 Da Isoelectric Point: 6.4674
>T6109 YP_009065961.1 NC_025100:627-932 [uncultured bacterium]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI
Download Length: 306 bp
>T6109 NC_025100:627-932 [uncultured bacterium]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA
Antitoxin
Download Length: 73 a.a. Molecular weight: 8372.34 Da Isoelectric Point: 4.7405
>AT6109 YP_009065960.1 NC_025100:407-625 [uncultured bacterium]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW
Download Length: 219 bp
>AT6109 NC_025100:407-625 [uncultured bacterium]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T6145 | Escherichia coli K-12 |
100 |
100 |
1 |
| T10064 | Dickeya dadantii 3937 |
36.275 |
100 |
0.366 |
| T190 | Escherichia coli O157:H7 str. EDL933 |
35.577 |
100 |
0.366 |
| T10018 | Escherichia coli O157:H7 str. Sakai |
35.577 |
100 |
0.366 |
| T10099 | Escherichia coli Nissle 1917 |
35.577 |
100 |
0.366 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT6145 | Escherichia coli K-12 |
100 |
100 |
1 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Daniel Wibberg et al. (2013) The IncF plasmid pRSB225 isolated from a municipal wastewater treatment plant's on-site preflooder combining antibiotic resistance and putative virulence functions is highly related to virulence plasmids identified in pathogenic E. coli isolates. Plasmid 69(2):127-37. [PubMed:23212116]