Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ccdAB/RHH-MazF |
| Location | 54803..55353 | Replicon | chromosome |
| Accession | NC_002695 | ||
| Organism | Escherichia coli O157:H7 str. Sakai | ||
Toxin (Protein)
| Gene name | ccdB | Uniprot ID | - |
| Locus tag | ECs_0053 | Protein ID | NP_308080.1 |
| Coordinates | 55039..55353 (+) | Length | 105 a.a. |
Antitoxin (Protein)
| Gene name | ccdA | Uniprot ID | - |
| Locus tag | ECs_0052 | Protein ID | NP_308079.1 |
| Coordinates | 54803..55036 (+) | Length | 78 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECs_0047 | 49877..50164 | + | 288 | NP_308074.1 | ferredoxin | - |
| ECs_0048 | 50222..51553 | + | 1332 | NP_308075.1 | MFS sugar transporter | - |
| ECs_0049 | 51661..52191 | + | 531 | NP_308076.1 | potassium transporter | - |
| ECs_0050 | 52184..54046 | + | 1863 | NP_308077.1 | glutathione-regulated potassium-efflux system protein | - |
| ECs_0051 | 54127..54717 | + | 591 | NP_308078.2 | dihydrofolate reductase | - |
| ECs_0052 | 54803..55036 | + | 234 | NP_308079.1 | CcdA-like protein | Antitoxin |
| ECs_0053 | 55039..55353 | + | 315 | NP_308080.1 | CcdB-like protein | Toxin |
| ECs_0054 | 55350..56198 | - | 849 | NP_308081.1 | diadenosine tetraphosphatase | - |
| ECs_0055 | 56205..56582 | - | 378 | NP_308082.1 | CO2+/MG2+ efflux protein ApaG | - |
| ECs_0056 | 56585..57406 | - | 822 | NP_308083.1 | ribosomal RNA small subunit methyltransferase A | - |
| ECs_0057 | 57403..58392 | - | 990 | NP_308084.1 | 4-hydroxy-L-threonine phosphate dehydrogenase | - |
| ECs_0058 | 58392..59678 | - | 1287 | NP_308085.1 | peptidyl-prolyl cis-trans isomerase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 105 a.a. Molecular weight: 11711.76 Da Isoelectric Point: 9.8517
>T10018 NP_308080.1 NC_002695:55039-55353 [Escherichia coli O157:H7 str. Sakai]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI
Download Length: 315 bp
>T10018 NC_002695:55039-55353 [Escherichia coli O157:H7 str. Sakai]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA
Antitoxin
Download Length: 78 a.a. Molecular weight: 8530.53 Da Isoelectric Point: 5.8062
>AT10018 NP_308079.1 NC_002695:54803-55036 [Escherichia coli O157:H7 str. Sakai]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF
Download Length: 234 bp
>AT10018 NC_002695:54803-55036 [Escherichia coli O157:H7 str. Sakai]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T190 | Escherichia coli O157:H7 str. EDL933 |
100 |
100 |
1 |
| T10099 | Escherichia coli Nissle 1917 |
98.077 |
100 |
0.981 |
| T10064 | Dickeya dadantii 3937 |
47.573 |
100 |
0.48 |
| T6145 | Escherichia coli K-12 |
35.577 |
100 |
0.366 |
| T6109 | uncultured bacterium |
35.577 |
100 |
0.366 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT190 | Escherichia coli O157:H7 str. EDL933 |
100 |
100 |
1 |
| AT10099 | Escherichia coli Nissle 1917 |
100 |
100 |
1 |
| AT10064 | Dickeya dadantii 3937 |
43.421 |
98.701 |
0.429 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Myriam Wilbaux et al. (2007) Functional interactions between coexisting toxin-antitoxin systems of the ccd family in Escherichia coli O157:H7. Journal of Bacteriology 189(7):2712-9. [PubMed:17259320]
(2) Jacob Bobonis et al. (2022) Bacterial retrons encode phage-defending tripartite toxin-antitoxin systems. Nature 609(7925):144-150. [PubMed:35850148]