Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) ccdAB/RHH-MazF
Location 54803..55353 Replicon chromosome
Accession NC_002655
Organism Escherichia coli O157:H7 str. EDL933

Toxin (Protein)


Gene name ccdB Uniprot ID D3H432
Locus tag Z_RS00275 Protein ID WP_001160975.1
Coordinates 55039..55353 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID V0VEX8
Locus tag Z_RS00270 Protein ID WP_000125566.1
Coordinates 54803..55036 (+) Length 78 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Z_RS00245 (Z0050) 49877..50164 + 288 WP_000203741.1 ferredoxin-like protein FixX -
Z_RS00250 (Z0051) 50222..51553 + 1332 WP_001183177.1 MFS transporter -
Z_RS00255 (Z0052) 51661..52191 + 531 WP_000600700.1 glutathione-regulated potassium-efflux system oxidoreductase KefF -
Z_RS00260 (Z0053) 52184..54046 + 1863 WP_000377197.1 glutathione-regulated potassium-efflux system protein KefC -
Z_RS00265 (Z0055) 54238..54717 + 480 WP_000624372.1 type 3 dihydrofolate reductase -
Z_RS00270 (Z0056) 54803..55036 + 234 WP_000125566.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
Z_RS00275 (Z0057) 55039..55353 + 315 WP_001160975.1 CcdB family protein Toxin
Z_RS00280 (Z0058) 55350..56198 - 849 WP_000257187.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH -
Z_RS00285 (Z0059) 56205..56582 - 378 WP_000610901.1 Co2+/Mg2+ efflux protein ApaG -
Z_RS00290 (Z0060) 56585..57406 - 822 WP_001065380.1 16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA -
Z_RS00295 (Z0061) 57403..58392 - 990 WP_000241259.1 4-hydroxythreonine-4-phosphate dehydrogenase PdxA -
Z_RS00300 (Z0062) 58392..59678 - 1287 WP_000800457.1 peptidylprolyl isomerase SurA -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-103)

Antitoxin

(8-77)


Sequences


Toxin        


Download         Length: 105 a.a.        Molecular weight: 11711.76 Da        Isoelectric Point: 9.8517

>T190 WP_001160975.1 NC_002655:55039-55353 [Escherichia coli O157:H7 str. EDL933]
MQFTVYRSRSRNAAFPFVIDVTSDIIGVINRRIVIPLTPIERFSRIRPPERLNPILLLVDGKEYVLMTHETATVPVNALG
TKFCDASAHRTLIKGALDFMLDGI

Download         Length: 315 bp

>T190 NC_002655:55039-55353 [Escherichia coli O157:H7 str. EDL933]
ATGCAATTTACGGTATACCGCAGTCGCAGCAGGAACGCCGCGTTTCCCTTTGTTATCGATGTTACCAGCGACATTATTGG
TGTGATTAATCGCCGTATTGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin


Download         Length: 78 a.a.        Molecular weight: 8530.53 Da        Isoelectric Point: 5.8062

>AT190 WP_000125566.1 NC_002655:54803-55036 [Escherichia coli O157:H7 str. EDL933]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Download         Length: 234 bp

>AT190 NC_002655:54803-55036 [Escherichia coli O157:H7 str. EDL933]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CTGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T10018 Escherichia coli O157:H7 str. Sakai

100

100

1

T10099 Escherichia coli Nissle 1917

98.077

100

0.981

T10064 Dickeya dadantii 3937

47.573

100

0.48

T6145 Escherichia coli K-12

35.577

100

0.366

T6109 uncultured bacterium

35.577

100

0.366


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT10099 Escherichia coli Nissle 1917

100

100

1

AT10018 Escherichia coli O157:H7 str. Sakai

100

100

1

AT10064 Dickeya dadantii 3937

43.421

98.701

0.429


Multiple sequence alignment    




Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LTM9

References


(1) Myriam Wilbaux et al. (2007) Functional interactions between coexisting toxin-antitoxin systems of the ccd family in Escherichia coli O157:H7. Journal of Bacteriology 189(7):2712-9. [PubMed:17259320] experimental

(2) Kritika Gupta et al. (2017) Contribution of the Chromosomal ccdAB Operon to Bacterial Drug Tolerance. Journal of Bacteriology 199(19):e00397-17. [PubMed:28674066] experimental


experimental experimental literature