Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 89542..90067 Replicon plasmid putative_pEcol1
Accession NZ_CP048440
Organism Escherichia coli strain NBRC 3301

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag GXM24_RS23015 Protein ID WP_001159868.1
Coordinates 89542..89847 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag GXM24_RS23020 Protein ID WP_000813634.1
Coordinates 89849..90067 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM24_RS23000 85452..86618 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
GXM24_RS23005 87206..87961 - 756 WP_000852146.1 replication initiation protein RepE -
GXM24_RS23010 88735..89541 - 807 WP_000016982.1 site-specific integrase -
GXM24_RS23015 89542..89847 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
GXM24_RS23020 89849..90067 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
GXM24_RS23025 90647..91735 + 1089 WP_000952217.1 transcriptional repressor PifC -
GXM24_RS23030 91737..93962 + 2226 WP_000698737.1 phage T7 exclusion protein -
GXM24_RS23035 94012..94911 - 900 WP_000963206.1 nucleotide-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..98786 98786


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T149491 WP_001159868.1 NZ_CP048440:c89847-89542 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T149491 NZ_CP064118:c3440869-3440444 [Yersinia pestis]
GTGGCCCAGTGGCGATGTAACCTGCGGGTATCCTGGCATACTCAACTCTTTTCTTTATTGGCACACGGCATATTGGTCAT
TTTAACCTTGGTCGCGCCCTGGCCCTTGGGTTATACCGCCCTATGGCTGGTATTGCTGACGTTAGTGGTGTTTGAGTGTA
TCCGCAGCCAGAAAAGGATTAAATCCTGTCAGGGGGAAATCAGGCTAAAACCTGGCAACCTGGTGCGATGGAAAAGGCAT
GAATGGACCGTGGTCAAGCCGCCGTGGATCACCCGCTATGGGGTATTATTACATTTACAACAAACAGGCGGCCACGCGAC
CCGAAAGCGTCTATGGCTGTCCGCTGACAGCATGTCTGAAGATGAGTGGCGGCAGCTATGCCTGTTGCTGCGGCATTCAT
TTGAATCTGACGATGGAACGATGTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT149491 WP_000813634.1 NZ_CP048440:c90067-89849 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT149491 NZ_CP064118:c3441116-3440850 [Yersinia pestis]
ATGGAAATTGATAATAAAGCCCGTATTCACTGGGCATGCCGCCGTGGTATGCGTGAACTTGATATCTCCATCATGCCGTT
TTTTGAATATGAATATGATAGCTTGAGCGATAACGAGAAACAGGCATTTATCCGCTTGCTTGAGTGTGATGACCCAGATT
TATTCAACTGGCTGATGAACCACGGGGAGCCGCAGGATAGCGAGCTATATCAGATGGTCAAACTGATTCAATCACGAAAT
AAAGCCCGTGGCCCAGTGGCGATGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References