Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 43817..44442 Replicon plasmid putative_pEcol1
Accession NZ_CP048440
Organism Escherichia coli strain NBRC 3301

Toxin (Protein)


Gene name vapC Uniprot ID -
Locus tag GXM24_RS22720 Protein ID WP_000911324.1
Coordinates 44044..44442 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID L4J1D2
Locus tag GXM24_RS22715 Protein ID WP_000450532.1
Coordinates 43817..44044 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXM24_RS22715 43817..44044 + 228 WP_000450532.1 toxin-antitoxin system antitoxin VapB Antitoxin
GXM24_RS22720 44044..44442 + 399 WP_000911324.1 tRNA(fMet)-specific endonuclease VapC Toxin
GXM24_RS22725 44451..46604 - 2154 WP_000009350.1 type IV conjugative transfer system coupling protein TraD -
GXM24_RS22730 46857..47588 - 732 WP_000850422.1 complement resistance protein TraT -
GXM24_RS22735 47613..48134 - 522 WP_000632670.1 conjugal transfer entry exclusion protein TraS -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..98786 98786


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-51)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14800.09 Da        Isoelectric Point: 7.8604

>T149486 WP_000911324.1 NZ_CP048440:44044-44442 [Escherichia coli]
MLKFMLDTNICIFTIKNKPASVRERFNLNQGRMCISSVTLMEVIYGAEKSQMPERNLAVIEGFVSRIDVLDYDAAAATHT
GQIRAELALQGRPVGPFDQMIAGHARSRGLIIVTNNTREFERVGGLRIEDWS

Download         Length: 399 bp

>T149486 NZ_CP064118:c1065517-1065314 [Yersinia pestis]
ATGACAAAAACTGACTACCTGATGCGTTTAAGAAAATGTACGACTATCGATACGCTAGAACGTGTAATTGAAAAAAATAA
GTACGAACTTTCTGATGATGAACTGGAATTGTTTTACTCAGCTGCAGACCATCGCCTGGCTGAACTAACGATGAATAAGC
TTTATGATAAAATTCCACCTACTGTATGGCAACATGTGAAATAG

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8512.55 Da        Isoelectric Point: 4.5358

>AT149486 WP_000450532.1 NZ_CP048440:43817-44044 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPENVKRVEVIAVGRTRIITPAGETWDEWFDGNSVSADFMDNREQPGMQERESF

Download         Length: 228 bp

>AT149486 NZ_CP064118:c1065934-1065566 [Yersinia pestis]
ATGGATGAGTACTCGCCTAAGCGGCATGACATTGCCCAACTGAAGTTTCTATGTGAAAGCTTGTATGATGAAGGTATTGC
AACTCTTGGCGACAGCCATCATGGCTGGATAAATGACCCAACGTCGGCCATAAACCTTCAACTCAATGATTTGATCGAGC
ATATAGCTTCATTTGTGATGAGTTTTAAAATAAAATACCCGGATGGAAGCCAGCTTTCCGAGTTAGTGGAAGAATATTTA
GACGACACATATACTCTGTTTAGTAGTTATGGAATCAATGATCCTGAATTGCGTCGCTGGCAGAAAACCAAAGAGCATTT
ATTCAGACTTTTCTCAGGGGAATACGTATGTACCCTGATGAAAACTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A829CHF5

References