Detailed information of TA system TA010211

Experimentally validated

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 4778..5026
• Replicon: chromosome
• Accession: M23008
• Organism: E.coli NADPH-sulfite reductase flavoprotein component (cysJ) NADPH-sulfite reductase hemoprotein component (cysI) and 3'
• T1TAdb ID: TA07127

Toxin (Protein)

• Gene name: hokX
• Uniprot ID: -
• Locus tag: -
• Protein ID: -
• Coordinates: 4874..5026 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	sokX
Locus tag	-
Coordinates	4778..4834 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Locus_0	285..2084	+	1800	AAA23650.1	NADPH-sulfite reducatase flavoprotein component	-
Locus_1	2084..3802	+	1719	AAA23651.1	NADPH-sulfite reductase hemoprotein component	-
Locus_2	3876..4610	+	735	AAA23652.1	3'-phosphoadenosine-5'-phosphosulfate sulfotransferase	-
-	4778..4834	-	57	-	-	Antitoxin
-	4874..5026	+	153	-	-	Toxin

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	5557..5697	140

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (22-45)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5674.79 Da
• Isoelectric Point: 8.1204

>T10211 - M23008:4874-5026 [Escherichia coli]
MLTKYALVAICCVLQYWDSRCMVGHSLCELSIRERGMEFKAVLAYESKK

Nucleotide

• Length: 153 bp

>T10211 M23008:4874-5026 [Escherichia coli]
ATGCTGACAAAATATGCCCTTGTGGCAATATAGTGCTGTGTTTTACAGTACTGGGATTCACGCTGCATGGTAGGCCACTC
GTTGTGTGAGTTGAGTATCAGAGAACGTGGTATGGAGTTTAAGGCAGTTCTCGCTTACGAATCGAAGAAGTAG

Antitoxin

Nucleotide

• Length: 57 bp

>AT10211 M23008:c4834-4778 [Escherichia coli]
GTTTGGGTTCGAACGTAAGCCTAGGTTGATAGAAATATCGCCTGGGGCTTTTGTGGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T10210	Hafnia alvei	40.909	89.796	0.367
T6369	Escherichia coli K-12	33.333	100	0.347
T10124	Erwinia amylovora ATCC 49946	45.833	75	0.344
T10039	Escherichia coli O157:H7 str. Sakai	34.091	89.796	0.306

Multiple sequence alignment

Structures

Antitoxin

References

(1) K Pedersen et al. (1999) Multiple hok genes on the chromosome of Escherichia coli. Molecular Microbiology 32(5):1090-102. [PubMed:10361310]


 experimental literature