Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 21144..21637 Replicon plasmid pHB37-1
Accession NZ_CP053081
Organism Escherichia coli strain HB37

Toxin (Protein)


Gene name TsxA Uniprot ID U9Y9W8
Locus tag HKW69_RS25620 Protein ID WP_001230709.1
Coordinates 21383..21637 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID U9Y4R8
Locus tag HKW69_RS25615 Protein ID WP_000151456.1
Coordinates 21144..21386 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKW69_RS25590 16781..17035 - 255 WP_000678527.1 hypothetical protein -
HKW69_RS25595 17080..18006 - 927 WP_001290416.1 hypothetical protein -
HKW69_RS25600 18238..19500 + 1263 WP_000608644.1 IS1380-like element ISEc9 family transposase -
HKW69_RS25605 19753..20628 + 876 WP_031942271.1 class A extended-spectrum beta-lactamase CTX-M-123 -
HKW69_RS25610 20675..21007 - 333 WP_001393253.1 WbuC family cupin fold metalloprotein -
HKW69_RS25615 21144..21386 - 243 WP_000151456.1 hypothetical protein Antitoxin
HKW69_RS25620 21383..21637 - 255 WP_001230709.1 hypothetical protein Toxin
HKW69_RS25625 21634..22176 - 543 WP_001083904.1 hypothetical protein -
HKW69_RS25630 22280..22795 - 516 WP_001025393.1 J domain-containing protein -
HKW69_RS25635 23199..23528 - 330 WP_000855119.1 hypothetical protein -
HKW69_RS25640 24343..24756 + 414 WP_000465036.1 hypothetical protein -
HKW69_RS25645 24758..25537 + 780 WP_001164198.1 site-specific integrase -
HKW69_RS25650 25717..26361 + 645 WP_001144036.1 ParA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaCTX-M-123 - 1..103328 103328
- flank IS/Tn blaCTX-M-123 - 18238..20628 2390


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 9942.47 Da        Isoelectric Point: 10.1206

>T155996 WP_001230709.1 NZ_CP053081:c21637-21383 [Escherichia coli]
MRKNKGRLTYYLEVIDKKYHFVKKISSYSKEFTDGKTKRTKRTLSELVFNESEVEAIDFTKNGLRPVDKNILLTMVKEYK
ESDA

Download         Length: 255 bp

>T155996 NZ_CP068823:c72487-72113 [Escherichia coli]
ATGAAAACATTACCCGATACACTCGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACTCTGCTCTC
CCGACTGCTGGGCCAGCATTACGGCCTTACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAATTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCACTTGTCCCCGCTCTCAGTTAATAAACAGTATTGATATCCTCCGGGCTCGCCGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGCATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9136.45 Da        Isoelectric Point: 7.2775

>AT155996 WP_000151456.1 NZ_CP053081:c21386-21144 [Escherichia coli]
MTIENTPENIKKLRKKIGLTQTECGEIFGVGLSTWQKKEAKTHNQLNLSKGEFEYLLLLAGEHPEYVLHKKAESRSPEEP

Download         Length: 243 bp

>AT155996 NZ_CP068823:c72945-72577 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCCGATGACAATCACGACCGTCCCTGGTGGGGACTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGATATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAGCTCATGCTCACCAACGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCCGAAATGAAAAATTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A3G9H8F0

Antitoxin

Source ID Structure
AlphaFold DB A0A3G9GNF5

References