71998

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 86327..86591 Replicon plasmid p13KWH46-2
Accession NZ_CP019252
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag BWI84_RS26905 Protein ID WP_001331364.1
Coordinates 86439..86591 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 86327..86384 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS26890 81566..83857 - 2292 WP_001289276.1 hypothetical protein -
BWI84_RS26895 83850..84920 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
BWI84_RS26900 84939..86147 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 86327..86384 - 58 NuclAT_0 - Antitoxin
- 86327..86384 - 58 NuclAT_0 - Antitoxin
- 86327..86384 - 58 NuclAT_0 - Antitoxin
- 86327..86384 - 58 NuclAT_0 - Antitoxin
BWI84_RS26905 86439..86591 + 153 WP_001331364.1 Hok/Gef family protein Toxin
BWI84_RS26910 86663..86914 - 252 WP_001291964.1 hypothetical protein -
BWI84_RS29195 87573..87749 - 177 WP_001054904.1 hypothetical protein -
BWI84_RS26915 88141..88350 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
BWI84_RS26920 88422..89072 - 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
BWI84_RS26925 89146..91314 - 2169 WP_021265679.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..123053 123053


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T71998 WP_001331364.1 NZ_CP019252:86439-86591 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T71998 NZ_CP019252:86439-86591 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 58 bp

>AT71998 NZ_CP019252:c86384-86327 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References