Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 2394..2941 Replicon plasmid pHB37-1
Accession NZ_CP053081
Organism Escherichia coli strain HB37

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag HKW69_RS25505 Protein ID WP_000970007.1
Coordinates 2660..2941 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag HKW69_RS25500 Protein ID WP_000079941.1
Coordinates 2394..2663 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKW69_RS25490 454..1485 + 1032 WP_000907873.1 replication initiation protein -
HKW69_RS25495 2181..2339 + 159 WP_000841008.1 hypothetical protein -
HKW69_RS25500 2394..2663 + 270 WP_000079941.1 hypothetical protein Antitoxin
HKW69_RS25505 2660..2941 + 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
HKW69_RS25510 2981..3829 + 849 WP_001057996.1 3'-5' exonuclease -
HKW69_RS25515 3946..4419 + 474 WP_001334658.1 hypothetical protein -
HKW69_RS25520 4896..5162 - 267 WP_000955263.1 hypothetical protein -
HKW69_RS25525 5162..5362 - 201 WP_063120186.1 hypothetical protein -
HKW69_RS25530 5380..6894 - 1515 Protein_8 Tn3-like element TnAs1 family transposase -
HKW69_RS25535 6960..7664 + 705 WP_001067855.1 IS6-like element IS26 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaCTX-M-123 - 1..103328 103328
- flank IS/Tn - - 6960..7664 704


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T155995 WP_000970007.1 NZ_CP053081:2660-2941 [Escherichia coli]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T155995 NZ_CP068822:c4977710-4977399 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT155995 WP_000079941.1 NZ_CP053081:2394-2663 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT155995 NZ_CP068822:c4977398-4977108 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References