Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 60088..60352 Replicon plasmid pJ31
Accession NZ_CP053788
Organism Escherichia coli isolate J31

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag HQN81_RS23830 Protein ID WP_001331364.1
Coordinates 60200..60352 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 60088..60145 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HQN81_RS23815 55327..57618 - 2292 WP_001289276.1 hypothetical protein -
HQN81_RS23820 57611..58681 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
HQN81_RS23825 58700..59908 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 60088..60145 - 58 NuclAT_0 - Antitoxin
- 60088..60145 - 58 NuclAT_0 - Antitoxin
- 60088..60145 - 58 NuclAT_0 - Antitoxin
- 60088..60145 - 58 NuclAT_0 - Antitoxin
HQN81_RS23830 60200..60352 + 153 WP_001331364.1 Hok/Gef family protein Toxin
HQN81_RS23835 60424..60675 - 252 WP_001291964.1 hypothetical protein -
HQN81_RS23840 61334..61510 - 177 WP_001054904.1 hypothetical protein -
HQN81_RS23845 61902..62111 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
HQN81_RS23850 62183..62833 - 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
HQN81_RS23855 62907..65075 - 2169 WP_021537350.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid dfrA17 / aadA5 / sul2 / blaCTX-M-1 - 1..108661 108661


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T157842 WP_001331364.1 NZ_CP053788:60200-60352 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T157842 NZ_CP069488:c3964205-3963855 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 58 bp

>AT157842 NZ_CP053788:c60145-60088 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References