Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 57464..57728 Replicon plasmid unnamed3
Accession NZ_CP081194
Organism Escherichia coli strain EC1722-1

Toxin (Protein)


Gene name pndA Uniprot ID E6BRV3
Locus tag K4F91_RS25465 Protein ID WP_001303307.1
Coordinates 57576..57728 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 57464..57526 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K4F91_RS25450 (52703) 52703..54994 - 2292 WP_072800778.1 F-type conjugative transfer protein TrbC -
K4F91_RS25455 (54987) 54987..56057 - 1071 WP_052895276.1 IncI1-type conjugal transfer protein TrbB -
K4F91_RS25460 (56076) 56076..57284 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- (57464) 57464..57526 - 63 NuclAT_0 - Antitoxin
- (57464) 57464..57526 - 63 NuclAT_0 - Antitoxin
- (57464) 57464..57526 - 63 NuclAT_0 - Antitoxin
- (57464) 57464..57526 - 63 NuclAT_0 - Antitoxin
K4F91_RS25465 (57576) 57576..57728 + 153 WP_001303307.1 Hok/Gef family protein Toxin
K4F91_RS25470 (57800) 57800..58051 - 252 WP_001291965.1 hypothetical protein -
- (58438) 58438..58489 - 52 NuclAT_1 - -
- (58438) 58438..58489 - 52 NuclAT_1 - -
- (58438) 58438..58489 - 52 NuclAT_1 - -
- (58438) 58438..58489 - 52 NuclAT_1 - -
K4F91_RS25475 (59329) 59329..59466 - 138 WP_049290858.1 hypothetical protein -
K4F91_RS25480 (59511) 59511..60737 - 1227 WP_052895285.1 RNA-guided endonuclease TnpB family protein -
K4F91_RS25485 (61379) 61379..61588 + 210 WP_052895278.1 HEAT repeat domain-containing protein -
K4F91_RS25490 (61660) 61660..62322 - 663 WP_052895279.1 plasmid IncI1-type surface exclusion protein ExcA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid sul2 / floR - 1..105553 105553
- flank IS/Tn - - 59511..60743 1232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5817.21 Da        Isoelectric Point: 8.7948

>T212722 WP_001303307.1 NZ_CP081194:57576-57728 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T212722 NZ_CP107335:56133-56444 [Klebsiella pneumoniae]
ATGCACGTAATTTCAAAAGAGCCTTTTGAGGAAGCGGCAAAGCGATATCCCAACGATTCGTTAGCCATTCGGGCGTTGTA
CCGCTTAGTGCGCGAGACGGACTTCTCTTCTCCAGCTGAGATGCTAACTCTGATACCGAGCCTGGATAATTTTAAGTACA
GGAATAAGTGGTGGGTTTTGGATGTAGGGGGCAACAACTTAAGGGTTATCGCCTATATCAATTTCGTAAATAAGCGCTTC
TACGTGAAGCACATCGCAACCCACGCTGATTACGACAAGTTGACCCGCTATTACAGGGAGAACAAAGAATGA

Antitoxin


Download         Length: 63 bp

>AT212722 NZ_CP081194:c57526-57464 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A634Q5L1

Antitoxin

Download structure file

References