Detailed information of TA system TA177063

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 60456..60720
• Replicon: plasmid unnamed476
• Accession: NZ_CP062873
• Organism: Escherichia coli strain Res13-Lact-PEA14-20

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: U9Y6M3
• Locus tag: IMP76_RS24750
• Protein ID: WP_001331364.1
• Coordinates: 60568..60720 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	60456..60513 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
IMP76_RS24735	55695..57986	-	2292	WP_001289276.1	hypothetical protein	-
IMP76_RS24740	57979..59049	-	1071	WP_000151583.1	IncI1-type conjugal transfer protein TrbB	-
IMP76_RS24745	59068..60276	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	60456..60513	-	58	NuclAT_0	-	Antitoxin
-	60456..60513	-	58	NuclAT_0	-	Antitoxin
-	60456..60513	-	58	NuclAT_0	-	Antitoxin
-	60456..60513	-	58	NuclAT_0	-	Antitoxin
IMP76_RS24750	60568..60720	+	153	WP_001331364.1	Hok/Gef family protein	Toxin
IMP76_RS24755	60792..61043	-	252	WP_001291964.1	hypothetical protein	-
IMP76_RS24760	61702..61878	-	177	WP_001054904.1	hypothetical protein	-
IMP76_RS24765	62270..62479	+	210	WP_000062603.1	HEAT repeat domain-containing protein	-
IMP76_RS24770	62551..63201	-	651	WP_001178506.1	plasmid IncI1-type surface exclusion protein ExcA	-
IMP76_RS24775	63275..65443	-	2169	WP_021537350.1	DotA/TraY family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_023329 Escherichia coli Conjugative plasmid TA006370, TA030448 1..104399 0.007	inside	Conjugative plasmid	dfrA17 / aadA5 / sul2 / blaCTX-M-1	-	1..110363	110363

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5775.13 Da
• Isoelectric Point: 8.7948

>T177063 WP_001331364.1 NZ_CP062873:60568-60720 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T177063 NZ_CP083066:c4679175-4678642 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 58 bp

>AT177063 NZ_CP062873:c60513-60456 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A5W9M382

Antitoxin