Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 57559..57823 Replicon plasmid pHB37-1
Accession NZ_CP053081
Organism Escherichia coli strain HB37

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag HKW69_RS25845 Protein ID WP_001331364.1
Coordinates 57671..57823 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 57559..57621 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
HKW69_RS25830 52798..55089 - 2292 WP_001289276.1 hypothetical protein -
HKW69_RS25835 55082..56152 - 1071 WP_000151582.1 IncI1-type conjugal transfer protein TrbB -
HKW69_RS25840 56171..57379 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 57559..57621 - 63 NuclAT_0 - Antitoxin
- 57559..57621 - 63 NuclAT_0 - Antitoxin
- 57559..57621 - 63 NuclAT_0 - Antitoxin
- 57559..57621 - 63 NuclAT_0 - Antitoxin
HKW69_RS25845 57671..57823 + 153 WP_001331364.1 Hok/Gef family protein Toxin
HKW69_RS25850 57895..58146 - 252 WP_001291964.1 hypothetical protein -
HKW69_RS25855 58805..58981 - 177 WP_001054897.1 hypothetical protein -
HKW69_RS25860 59373..59582 + 210 WP_039025903.1 HEAT repeat domain-containing protein -
HKW69_RS25865 59654..60316 - 663 WP_000644796.1 plasmid IncI1-type surface exclusion protein ExcA -
HKW69_RS25870 60387..62555 - 2169 WP_000698354.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid blaCTX-M-123 - 1..103328 103328


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T155997 WP_001331364.1 NZ_CP053081:57671-57823 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T155997 NZ_CP068823:210288-210473 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 63 bp

>AT155997 NZ_CP053081:c57621-57559 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References