177093

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 15193..15740 Replicon plasmid unnamed476
Accession NZ_CP062877
Organism Escherichia coli O157:H16 strain Res13-Lact-PEA12-26

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag IMP69_RS23445 Protein ID WP_000970007.1
Coordinates 15459..15740 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag IMP69_RS23440 Protein ID WP_000079941.1
Coordinates 15193..15462 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IMP69_RS23410 10867..11622 + 756 Protein_11 IS110-like element IS5075 family transposase -
IMP69_RS23415 11804..11980 - 177 WP_000954592.1 Arm DNA-binding domain-containing protein -
IMP69_RS23420 12310..13125 + 816 WP_001043260.1 sulfonamide-resistant dihydropteroate synthase Sul2 -
IMP69_RS23425 13212..13454 + 243 Protein_14 phosphoglucosamine mutase -
IMP69_RS23430 13408..13659 - 252 WP_001445143.1 hypothetical protein -
IMP69_RS23435 13690..14826 - 1137 WP_023140202.1 IS91 family transposase -
IMP69_RS23440 15193..15462 + 270 WP_000079941.1 hypothetical protein Antitoxin
IMP69_RS23445 15459..15740 + 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
IMP69_RS23450 15780..16628 + 849 WP_021265674.1 3'-5' exonuclease -
IMP69_RS23455 16745..17218 + 474 WP_001334658.1 hypothetical protein -
IMP69_RS23460 17695..17961 - 267 WP_000955263.1 hypothetical protein -
IMP69_RS23465 17961..18455 - 495 WP_001459505.1 hypothetical protein -
IMP69_RS23470 18511..19113 - 603 WP_000517695.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid dfrA17 / aadA5 / sul2 / blaCTX-M-1 - 1..109586 109586


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T177093 WP_000970007.1 NZ_CP062877:15459-15740 [Escherichia coli O157:H16]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T177093 NZ_CP083075:c4850583-4850050 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT177093 WP_000079941.1 NZ_CP062877:15193-15462 [Escherichia coli O157:H16]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT177093 NZ_CP083075:c4850860-4850594 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References