Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 62954..63218 Replicon plasmid pCFS3313-2
Accession NZ_CP026941
Organism Escherichia coli strain CFS3313

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag C5F74_RS24770 Protein ID WP_001331364.1
Coordinates 63066..63218 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 62954..63011 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C5F74_RS24755 58193..60484 - 2292 WP_001289276.1 hypothetical protein -
C5F74_RS24760 60477..61547 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
C5F74_RS24765 61566..62774 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- 62954..63011 - 58 NuclAT_0 - Antitoxin
- 62954..63011 - 58 NuclAT_0 - Antitoxin
- 62954..63011 - 58 NuclAT_0 - Antitoxin
- 62954..63011 - 58 NuclAT_0 - Antitoxin
C5F74_RS24770 63066..63218 + 153 WP_001331364.1 Hok/Gef family protein Toxin
C5F74_RS24775 63290..63541 - 252 WP_001291964.1 hypothetical protein -
C5F74_RS25320 64200..64376 - 177 WP_001054904.1 hypothetical protein -
C5F74_RS24780 64768..64977 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
C5F74_RS24785 65049..65699 - 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
C5F74_RS24790 65773..67941 - 2169 WP_021537350.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid tet(A) / sul2 / blaCTX-M-1 - 1..111813 111813


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T96768 WP_001331364.1 NZ_CP026941:63066-63218 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T96768 NZ_CP026941:63066-63218 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 58 bp

>AT96768 NZ_CP026941:c63011-62954 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References