Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type I Classification (family/domain) hok-sok/-
Location 59789..60053 Replicon plasmid pKFu022_1
Accession NZ_CP091678
Organism Escherichia coli strain KFu022

Toxin (Protein)


Gene name pndA Uniprot ID U9Y6M3
Locus tag L6L66_RS23155 Protein ID WP_001331364.1
Coordinates 59901..60053 (+) Length 51 a.a.

Antitoxin (RNA)


Gene name pndB
Locus tag -
Coordinates 59789..59846 (-)

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
L6L66_RS23140 (55028) 55028..57319 - 2292 WP_001289276.1 F-type conjugative transfer protein TrbC -
L6L66_RS23145 (57312) 57312..58382 - 1071 WP_000151583.1 IncI1-type conjugal transfer protein TrbB -
L6L66_RS23150 (58401) 58401..59609 - 1209 WP_000121273.1 IncI1-type conjugal transfer protein TrbA -
- (59789) 59789..59846 - 58 NuclAT_0 - Antitoxin
- (59789) 59789..59846 - 58 NuclAT_0 - Antitoxin
- (59789) 59789..59846 - 58 NuclAT_0 - Antitoxin
- (59789) 59789..59846 - 58 NuclAT_0 - Antitoxin
L6L66_RS23155 (59901) 59901..60053 + 153 WP_001331364.1 Hok/Gef family protein Toxin
L6L66_RS23160 (60125) 60125..60376 - 252 WP_001291964.1 hypothetical protein -
L6L66_RS24210 (60875) 60875..60970 + 96 WP_001303310.1 DinQ-like type I toxin DqlB -
L6L66_RS23170 (61035) 61035..61211 - 177 WP_001054904.1 hypothetical protein -
L6L66_RS23175 (61603) 61603..61812 + 210 WP_000062603.1 HEAT repeat domain-containing protein -
L6L66_RS23180 (61884) 61884..62534 - 651 WP_001178506.1 plasmid IncI1-type surface exclusion protein ExcA -
L6L66_RS23185 (62608) 62608..64776 - 2169 WP_021537350.1 DotA/TraY family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid tet(A) / sul2 / blaCTX-M-1 - 1..108771 108771


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Hok/gef cell toxic protein

(7-46)


Sequences

Toxin        


Download         Length: 51 a.a.        Molecular weight: 5775.13 Da        Isoelectric Point: 8.7948

>T234483 WP_001331364.1 NZ_CP091678:59901-60053 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCGLRLQQGNTVLVATLAYEVKR

Download         Length: 153 bp

>T234483 NZ_LR130545:c4335866-4335579 [Escherichia coli]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCCAAAAGTAATCGGGTGAAACATGGCATCCGCTTTGAAGATGCGGT
GTTAGTGTTTGATGATCCACAACATTTGTCACAGCAAGATCGCATAGAAAACGGTGAGTATCGCTGGCAGACTATCGGCC
TGGTTCACGGTATCGTGGTTATTCTGGTTGCGCATACCATCCGTTTCGAAAGCGGAAATGAAATTATTCGAATCATCAGT
GCACGAAAAGCAGATCGTAAAGAGAGGAGCCGCTATGAGCATGGTTAA

Antitoxin


Download         Length: 58 bp

>AT234483 NZ_CP091678:c59846-59789 [Escherichia coli]
GCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5W9M382

Antitoxin

Download structure file

References