71997

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 39183..39730 Replicon plasmid p13KWH46-2
Accession NZ_CP019252
Organism Escherichia coli strain 13KWH46

Toxin (Protein)


Gene name parE Uniprot ID W0LWB3
Locus tag BWI84_RS26580 Protein ID WP_000970007.1
Coordinates 39449..39730 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID E1QMW3
Locus tag BWI84_RS26575 Protein ID WP_000079941.1
Coordinates 39183..39452 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BWI84_RS29165 35042..35254 + 213 WP_021265676.1 hypothetical protein -
BWI84_RS26540 35369..36577 - 1209 WP_000198533.1 IS91 family transposase -
BWI84_RS26545 36558..36830 - 273 WP_000019163.1 hypothetical protein -
BWI84_RS26555 36912..37115 + 204 Protein_39 dihydropteroate synthase -
BWI84_RS26560 37202..37444 + 243 Protein_40 phosphoglucosamine mutase -
BWI84_RS26570 37680..38825 - 1146 Protein_41 IS91-like element ISVsa3 family transposase -
BWI84_RS26575 39183..39452 + 270 WP_000079941.1 hypothetical protein Antitoxin
BWI84_RS26580 39449..39730 + 282 WP_000970007.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BWI84_RS26585 39770..40618 + 849 WP_021265674.1 3'-5' exonuclease -
BWI84_RS26590 40735..41208 + 474 WP_001334658.1 hypothetical protein -
BWI84_RS29170 41685..41951 - 267 WP_000955263.1 hypothetical protein -
BWI84_RS26610 43353..43781 - 429 WP_148116993.1 hypothetical protein -
BWI84_RS26615 43837..44439 - 603 WP_000517695.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Conjugative plasmid - - 1..123053 123053
- inside IScluster/Tn - - 35369..43264 7895


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10892.63 Da        Isoelectric Point: 9.7617

>T71997 WP_000970007.1 NZ_CP019252:39449-39730 [Escherichia coli]
MMEIFWTMLASQDRKRIRKYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YILRVLHTAQKWP

Download         Length: 282 bp

>T71997 NZ_CP019252:39449-39730 [Escherichia coli]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTAAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGCCGCAACGGTC
GTGTGGAAGGTACCCGGGAACTGGTTATTCACCCGCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATATCCTGCGCGTGTTGCATACTGCACAGAAGTGGCCATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10252.58 Da        Isoelectric Point: 7.2057

>AT71997 WP_000079941.1 NZ_CP019252:39183-39452 [Escherichia coli]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLERGDVLPHDMVESSAAAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT71997 NZ_CP019252:39183-39452 [Escherichia coli]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATTCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCGCGGCGATGTGCTTCCCCATGACATGGTCGAATCTTCCGCTGCTGCATGGCGGGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7G1GDN5

Antitoxin

Source ID Structure
AlphaFold DB A0A629TXC5

References