23740

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 461732..462291 Replicon chromosome II
Accession NC_012583
Organism Vibrio cholerae O395

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag VC395_RS16710 Protein ID WP_000578476.1
Coordinates 461732..462010 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag VC395_RS16715 Protein ID WP_000381183.1
Coordinates 462007..462291 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VC395_RS16665 457032..457469 + 438 WP_000503164.1 IS200/IS605-like element IS1004 family transposase -
VC395_RS16670 457533..457751 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VC395_RS16675 457926..458798 + 873 WP_000254716.1 DUF3800 domain-containing protein -
VC395_RS16680 458948..459547 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
VC395_RS20935 459604..459708 + 105 WP_099607150.1 acetyltransferase -
VC395_RS16685 459739..460008 + 270 WP_001114075.1 DUF4160 domain-containing protein -
VC395_RS16690 460002..460523 + 522 WP_000921691.1 DUF2442 domain-containing protein -
VC395_RS20685 460822..460947 + 126 WP_001905700.1 DUF645 family protein -
VC395_RS16705 461198..461593 + 396 WP_001000867.1 hypothetical protein -
VC395_RS16710 461732..462010 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
VC395_RS16715 462007..462291 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
VC395_RS16720 462485..463000 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
VC395_RS16725 463184..463597 + 414 WP_000049420.1 VOC family protein -
VC395_RS16735 464152..465329 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -
VC395_RS16740 465409..465678 - 270 WP_000402869.1 hypothetical protein -
VC395_RS16745 465692..466636 - 945 WP_001027829.1 hypothetical protein -
VC395_RS16750 466753..467160 - 408 WP_000022491.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Prophage - - 427343..471178 43835
- inside Integron - - 450510..464084 13574
flank IS/Tn - - 457032..457469 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T23740 WP_000578476.1 NC_012583:c462010-461732 [Vibrio cholerae O395]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T23740 NC_012583:c462010-461732 [Vibrio cholerae O395]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT23740 WP_000381183.1 NC_012583:c462291-462007 [Vibrio cholerae O395]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT23740 NC_012583:c462291-462007 [Vibrio cholerae O395]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9K2M8

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References