Detailed information of TA system TA142369

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): dhiT-phd/-
• Location: 803483..803887
• Replicon: chromosome
• Accession: NZ_CP045718
• Organism: Vibrio cholerae O395 substr. TCP2

Toxin (Protein)

• Gene name: dhiT
• Uniprot ID: A0A086SLD6
• Locus tag: GG844_RS03555
• Protein ID: WP_001114075.1
• Coordinates: 803483..803752 (-)
• Length: 90 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: GG844_RS03560
• Protein ID: WP_099607150.1
• Coordinates: 803783..803887 (-)
• Length: 35 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GG844_RS03515	799894..800307	-	414	WP_000049420.1	VOC family protein	-
GG844_RS03520	800491..801006	-	516	WP_000343779.1	GNAT family N-acetyltransferase	-
GG844_RS03525	801200..801484	+	285	WP_000381183.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	-
GG844_RS03530	801481..801759	+	279	WP_000578476.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	-
GG844_RS03535	801898..802293	-	396	WP_001000867.1	hypothetical protein	-
GG844_RS03545	802544..802669	-	126	WP_001905700.1	DUF645 family protein	-
GG844_RS03550	802968..803489	-	522	WP_000921691.1	DUF2442 domain-containing protein	-
GG844_RS03555	803483..803752	-	270	WP_001114075.1	DUF4160 domain-containing protein	Toxin
GG844_RS03560	803783..803887	-	105	WP_099607150.1	acetyltransferase	Antitoxin
GG844_RS03565	803944..804543	-	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
GG844_RS03570	804693..805565	-	873	WP_000254716.1	DUF3800 domain-containing protein	-
GG844_RS03575	805740..805958	-	219	WP_071908318.1	GNAT family N-acetyltransferase	-
GG844_RS03580	806022..806459	-	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
GG844_RS03585	806578..806787	-	210	Protein_694	GNAT family N-acetyltransferase	-
GG844_RS03590	806923..807312	-	390	WP_001081302.1	hypothetical protein	-
GG844_RS03595	807487..807729	-	243	WP_000107462.1	hypothetical protein	-
GG844_RS03600	807937..808854	-	918	WP_000186333.1	alpha/beta hydrolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	791552..816242	24690
	-	inside	Integron	-	-	799407..812981	13574
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0.002	flank	IS/Tn	-	-	806022..806459	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF4160 (9-74)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 90 a.a.
• Molecular weight: 10310.94 Da
• Isoelectric Point: 6.6303

>T142369 WP_001114075.1 NZ_CP045718:c803752-803483 [Vibrio cholerae O395]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Nucleotide

• Length: 270 bp

>T142369 NZ_CP060672:c1462565-1462347 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 35 a.a.
• Molecular weight: 3991.79 Da
• Isoelectric Point: 9.2853

>AT142369 WP_099607150.1 NZ_CP045718:c803887-803783 [Vibrio cholerae O395]
VVSVVVFEFSSMRCQPLRRALYAYRNCLLKDTLL

Nucleotide

• Length: 105 bp

>AT142369 NZ_CP060672:c1462967-1462593 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: A0A086SLD6