23718

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/ParE-Phd
Location 401321..401868 Replicon chromosome II
Accession NC_012580
Organism Vibrio cholerae M66-2

Toxin (Protein)


Gene name relE Uniprot ID Q9KM92
Locus tag VCM66_RS15390 Protein ID WP_000229317.1
Coordinates 401566..401868 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9KM93
Locus tag VCM66_RS15385 Protein ID WP_000861987.1
Coordinates 401321..401578 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCM66_RS15345 396350..396805 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
VCM66_RS19005 397127..397279 + 153 WP_001884520.1 DUF645 family protein -
VCM66_RS15350 397481..397978 - 498 WP_000982260.1 GNAT family N-acetyltransferase -
VCM66_RS15355 397975..398247 - 273 WP_000246253.1 DUF1778 domain-containing protein -
VCM66_RS15360 398478..399146 + 669 WP_000043871.1 hypothetical protein -
VCM66_RS15365 399298..399585 + 288 WP_000426470.1 hypothetical protein -
VCM66_RS15370 399726..400097 + 372 WP_001164080.1 nucleotide pyrophosphohydrolase -
VCM66_RS19595 400164..400589 + 426 WP_001882332.1 DUF1778 domain-containing protein -
VCM66_RS15380 400586..401119 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
VCM66_RS15385 401321..401578 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
VCM66_RS15390 401566..401868 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
VCM66_RS15395 402022..402231 + 210 Protein_419 GNAT family N-acetyltransferase -
VCM66_RS15400 402350..402787 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
VCM66_RS15405 402851..403069 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VCM66_RS15410 403244..404116 + 873 WP_000254716.1 DUF3800 domain-containing protein -
VCM66_RS15415 404266..404865 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
VCM66_RS19440 404922..405026 + 105 WP_099607150.1 acetyltransferase -
VCM66_RS15420 405057..405326 + 270 WP_001114075.1 DUF4160 domain-containing protein -
VCM66_RS15425 405320..405841 + 522 WP_000921691.1 DUF2442 domain-containing protein -
VCM66_RS19445 406140..406265 + 126 WP_001905700.1 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 305869..409778 103909
- inside Integron - - 310949..409402 98453
flank IS/Tn - - 402350..402787 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-69)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11726.68 Da        Isoelectric Point: 5.1972

>T23718 WP_000229317.1 NC_012580:401566-401868 [Vibrio cholerae M66-2]
MVEIIWTELALSDLNDIAEYIALENVVAAKQLVQTVFTKVERLADFPESGRVPPELEHLNYREVVVSPCRVFYKYDDAKV
RILFVMRAERDLRRLMLTKQ

Download         Length: 303 bp

>T23718 NC_012580:401566-401868 [Vibrio cholerae M66-2]
ATGGTTGAAATAATTTGGACGGAGCTGGCTCTATCCGACTTGAATGATATTGCTGAATACATCGCGCTTGAAAATGTCGT
GGCTGCTAAACAACTGGTGCAAACCGTTTTTACAAAAGTTGAACGTTTGGCCGATTTTCCAGAGTCTGGGCGCGTCCCTC
CAGAACTAGAACACCTCAATTATCGTGAAGTCGTTGTAAGTCCGTGTCGTGTTTTCTACAAGTATGATGATGCAAAGGTT
CGTATTCTTTTTGTTATGCGTGCGGAGCGAGATTTGCGTCGGTTAATGCTTACGAAACAGTAG

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9647.15 Da        Isoelectric Point: 7.3178

>AT23718 WP_000861987.1 NC_012580:401321-401578 [Vibrio cholerae M66-2]
MKVELVTSLKRQATKILADLHDTKEPVLITEHGKPSAYLIDVDDYEFMQNRLAILEGIARGERALADGKVVSHQDAKDRM
SKWLK

Download         Length: 258 bp

>AT23718 NC_012580:401321-401578 [Vibrio cholerae M66-2]
ATGAAAGTAGAACTTGTGACGTCACTAAAACGTCAGGCCACTAAGATCCTTGCCGATCTTCATGATACGAAAGAGCCAGT
ATTGATAACTGAGCACGGAAAACCGTCAGCTTACCTTATTGATGTAGACGACTATGAATTTATGCAAAATCGTTTAGCGA
TCCTGGAAGGTATTGCTCGCGGAGAGCGAGCTTTAGCGGATGGTAAAGTGGTCAGCCATCAAGATGCTAAGGACAGAATG
TCAAAATGGTTGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9KM92

Antitoxin

Source ID Structure
AlphaFold DB A0A1T4SLM9

References