23720

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DhiAT/-
Location 405057..405841 Replicon chromosome II
Accession NC_012580
Organism Vibrio cholerae M66-2

Toxin (Protein)


Gene name dhiT Uniprot ID A0A086SLD6
Locus tag VCM66_RS15420 Protein ID WP_001114075.1
Coordinates 405057..405326 (+) Length 90 a.a.

Antitoxin (Protein)


Gene name dhiA Uniprot ID Q9KM84
Locus tag VCM66_RS15425 Protein ID WP_000921691.1
Coordinates 405320..405841 (+) Length 174 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
VCM66_RS19595 400164..400589 + 426 WP_001882332.1 DUF1778 domain-containing protein -
VCM66_RS15380 400586..401119 + 534 WP_000118351.1 GNAT family N-acetyltransferase -
VCM66_RS15385 401321..401578 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
VCM66_RS15390 401566..401868 + 303 WP_000229317.1 type II toxin-antitoxin system RelE/ParE family toxin -
VCM66_RS15395 402022..402231 + 210 Protein_419 GNAT family N-acetyltransferase -
VCM66_RS15400 402350..402787 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
VCM66_RS15405 402851..403069 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
VCM66_RS15410 403244..404116 + 873 WP_000254716.1 DUF3800 domain-containing protein -
VCM66_RS15415 404266..404865 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
VCM66_RS19440 404922..405026 + 105 WP_099607150.1 acetyltransferase -
VCM66_RS15420 405057..405326 + 270 WP_001114075.1 DUF4160 domain-containing protein Toxin
VCM66_RS15425 405320..405841 + 522 WP_000921691.1 DUF2442 domain-containing protein Antitoxin
VCM66_RS19445 406140..406265 + 126 WP_001905700.1 DUF645 family protein -
VCM66_RS15430 406516..406911 + 396 WP_001000867.1 hypothetical protein -
VCM66_RS15435 407050..407328 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family -
VCM66_RS15440 407325..407609 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin -
VCM66_RS15445 407803..408318 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
VCM66_RS15450 408502..408915 + 414 WP_000049420.1 VOC family protein -
VCM66_RS15460 409470..410647 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 305869..409778 103909
- inside Integron - - 310949..409402 98453
flank IS/Tn - - 402350..402787 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Protein of unknown function DUF4160

(9-74)

Antitoxin

Protein of unknown function DUF2442

(4-59)


Sequences

Toxin        


Download         Length: 90 a.a.        Molecular weight: 10310.94 Da        Isoelectric Point: 6.6303

>T23720 WP_001114075.1 NC_012580:405057-405326 [Vibrio cholerae M66-2]
MPEIDALLGLSFCLYFFDNKQHKLPHIHVKYGSYELIIAIETGECLEGYLPNKQRKRAENHILEHREQLMVMWNKAVNGE
NPGKLGDLC

Download         Length: 270 bp

>T23720 NC_012580:405057-405326 [Vibrio cholerae M66-2]
ATGCCAGAGATTGATGCCCTATTAGGTCTTTCATTTTGCTTGTACTTCTTTGATAACAAGCAGCATAAATTACCTCATAT
TCACGTTAAGTACGGTAGTTACGAGCTAATTATTGCTATTGAAACAGGTGAGTGTTTAGAGGGTTACTTACCCAATAAGC
AACGAAAACGTGCTGAAAACCATATTCTTGAACATCGAGAGCAATTGATGGTGATGTGGAATAAAGCGGTGAATGGTGAA
AATCCAGGTAAGTTAGGTGATTTATGTTGA

Antitoxin


Download         Length: 174 a.a.        Molecular weight: 19409.01 Da        Isoelectric Point: 6.7342

>AT23720 WP_000921691.1 NC_012580:405320-405841 [Vibrio cholerae M66-2]
MLKVIDVDFVSDHTLELTFNDGYQGYADLSVYFKKAPFSEIKDFKRFSLTRDGSLNWDGNELTAATLRDITKGSQKSVEL
SFNVQEMEAVIKQASWESMMEGRPDILQAAIRSYVEQFGHGQVIAKAGIKSRTSAYRSLKPETTPNFGTLVQLGHAVIEL
AKDRTVANKEPRI

Download         Length: 522 bp

>AT23720 NC_012580:405320-405841 [Vibrio cholerae M66-2]
ATGTTGAAAGTCATAGATGTTGATTTTGTCTCAGACCATACATTAGAACTGACGTTCAATGATGGTTACCAAGGCTATGC
CGACTTGTCGGTATACTTTAAAAAAGCACCGTTTTCAGAAATTAAGGACTTTAAACGCTTTTCCTTGACTCGTGATGGCT
CGTTAAATTGGGATGGTAATGAGTTAACGGCCGCGACACTCCGTGACATAACCAAGGGCTCACAAAAGTCGGTGGAGTTA
AGTTTTAATGTTCAAGAAATGGAAGCCGTCATCAAACAGGCCTCATGGGAATCGATGATGGAGGGGCGTCCCGACATCTT
GCAAGCGGCTATTCGTTCTTACGTAGAGCAGTTTGGTCATGGTCAAGTTATTGCAAAGGCGGGTATAAAAAGTCGTACGA
GTGCTTATCGCTCCTTGAAGCCAGAAACAACGCCAAACTTTGGCACACTAGTTCAGTTAGGGCACGCCGTCATCGAACTA
GCGAAAGATAGAACAGTGGCAAACAAGGAACCACGCATATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A086SLD6

Antitoxin

Source ID Structure
AlphaFold DB Q9KM84

References