119901

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /GNAT-DUF1778
Location 381109..381875 Replicon chromosome
Accession NZ_CP036500
Organism Vibrio cholerae strain 20000

Toxin (Protein)


Gene name - Uniprot ID Q9K2P7
Locus tag EZZ25_RS15920 Protein ID WP_000982260.1
Coordinates 381109..381606 (-) Length 166 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID Q9K2J6
Locus tag EZZ25_RS15925 Protein ID WP_000246253.1
Coordinates 381603..381875 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EZZ25_RS15865 376533..376703 + 171 WP_000483444.1 DUF645 family protein -
EZZ25_RS15870 376882..377542 + 661 Protein_366 Vat family streptogramin A O-acetyltransferase -
EZZ25_RS15875 377679..378185 + 507 Protein_367 GNAT family N-acetyltransferase -
EZZ25_RS15880 378304..378741 + 438 WP_000503164.1 IS200/IS605-like element IS1004 family transposase -
EZZ25_RS15885 378823..378993 + 171 WP_001080654.1 hypothetical protein -
EZZ25_RS15890 378993..379394 + 402 Protein_370 type II toxin-antitoxin system death-on-curing family toxin -
EZZ25_RS15895 379364..379507 + 144 WP_071908341.1 DUF3265 domain-containing protein -
EZZ25_RS15900 379592..379843 + 252 WP_088124792.1 TIGR03643 family protein -
EZZ25_RS15905 379977..380432 + 456 WP_001245327.1 GNAT family N-acetyltransferase -
EZZ25_RS15910 380754..380906 + 153 WP_001884520.1 DUF645 family protein -
EZZ25_RS15920 381109..381606 - 498 WP_000982260.1 GNAT family N-acetyltransferase Toxin
EZZ25_RS15925 381603..381875 - 273 WP_000246253.1 DUF1778 domain-containing protein Antitoxin
EZZ25_RS15930 382106..382774 + 669 WP_000043871.1 hypothetical protein -
EZZ25_RS15935 382926..383213 + 288 WP_000426470.1 hypothetical protein -
EZZ25_RS19430 383373..383477 + 105 Protein_379 PhzF family phenazine biosynthesis protein -
EZZ25_RS15940 383535..383969 + 435 WP_000453958.1 GNAT family N-acetyltransferase -
EZZ25_RS15955 384124..384627 + 504 WP_001898452.1 hypothetical protein -
EZZ25_RS19355 384819..385322 + 504 WP_001898452.1 hypothetical protein -
EZZ25_RS15970 385550..385807 + 258 WP_000861987.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
EZZ25_RS15975 385795..386097 + 303 WP_000229325.1 type II toxin-antitoxin system RelE/ParE family toxin -
EZZ25_RS15985 386482..386592 + 111 WP_076025930.1 DUF645 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
flank IS/Tn - - 378304..378741 437
- inside Integron - - 322986..403614 80628


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(86-152)

Antitoxin

Vibrio phage ICP1, Orf50

(7-86)


Sequences

Toxin        


Download         Length: 166 a.a.        Molecular weight: 18527.18 Da        Isoelectric Point: 8.7753

>T119901 WP_000982260.1 NZ_CP036500:c381606-381109 [Vibrio cholerae]
MMNTVLLDKDKHDRNRFNCGTEALNNYLKVMASQQAKKDNTRTFVLEDDNNSAYIIGFYTLTMTPIDLKALPDKLQKKHQ
SSTSGGLIARLAVDDRYKGKGFGEWLLIDALRKLLAASDSVAFPVVIVDAKDGAKHFYERYGFQAFQDAENKLFITIADI
RASLG

Download         Length: 498 bp

>T119901 NZ_CP036500:c381606-381109 [Vibrio cholerae]
ATGATGAATACGGTACTTCTCGATAAAGATAAGCACGATAGGAACCGTTTCAATTGTGGCACTGAAGCACTCAACAATTA
CTTAAAAGTAATGGCGAGTCAGCAAGCCAAAAAAGACAACACCAGAACCTTCGTTTTGGAAGATGACAATAACAGTGCTT
ATATCATCGGCTTTTACACTCTGACTATGACGCCCATTGATTTGAAAGCCTTGCCCGACAAGTTACAAAAGAAGCACCAA
TCCTCAACCTCTGGAGGGCTCATTGCTCGTTTAGCTGTCGACGATAGATACAAAGGAAAAGGTTTTGGTGAGTGGCTACT
CATTGACGCTCTCAGAAAGCTACTCGCAGCCAGTGACAGTGTAGCGTTCCCTGTTGTCATTGTTGATGCCAAAGATGGTG
CAAAACATTTCTATGAGCGCTATGGGTTTCAAGCGTTTCAAGATGCCGAAAACAAACTCTTCATCACCATTGCTGATATC
AGAGCAAGCTTAGGCTAG

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 9683.22 Da        Isoelectric Point: 6.2988

>AT119901 WP_000246253.1 NZ_CP036500:c381875-381603 [Vibrio cholerae]
MATTLPRITARVDVDTQDLLAKAAALAGMSSINSFVLNAAIEKAKQVIEREQALKLSQADAVLLMEALDNPAVVNAKLKL
ASERYESKTQ

Download         Length: 273 bp

>AT119901 NZ_CP036500:c381875-381603 [Vibrio cholerae]
ATGGCAACGACTTTGCCTCGCATCACCGCTAGAGTTGATGTCGATACTCAAGATTTACTCGCTAAGGCTGCTGCTCTTGC
TGGTATGTCTAGTATTAACTCATTTGTTTTGAATGCAGCAATCGAAAAAGCAAAACAAGTCATTGAACGTGAGCAAGCTC
TAAAGCTTAGCCAAGCTGATGCCGTATTGCTGATGGAAGCTCTTGATAATCCAGCTGTAGTAAATGCAAAGCTCAAATTG
GCATCCGAACGTTATGAGAGCAAAACTCAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0Q0KGB1

Antitoxin

Source ID Structure
AlphaFold DB A0A0F2IC79

References