30651

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 442853..443412 Replicon chromosome
Accession NZ_AP014525
Organism Vibrio cholerae MS6

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag MS6_RS15935 Protein ID WP_000578476.1
Coordinates 442853..443131 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag MS6_RS15940 Protein ID WP_000381183.1
Coordinates 443128..443412 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
MS6_RS15900 438153..438590 + 438 WP_041051975.1 IS200/IS605 family transposase -
MS6_RS15905 438654..438872 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
MS6_RS15910 439047..439919 + 873 WP_000254716.1 DUF3800 domain-containing protein -
MS6_RS15915 440069..440668 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
MS6_RS20255 440725..440829 + 105 WP_099607150.1 acetyltransferase -
MS6_RS15920 440860..441129 + 270 WP_001114075.1 DUF4160 domain-containing protein -
MS6_RS15925 441123..441644 + 522 WP_000921691.1 DUF2442 domain-containing protein -
MS6_RS20260 441943..442068 + 126 WP_001905700.1 DUF645 family protein -
MS6_RS15930 442319..442714 + 396 WP_001000867.1 hypothetical protein -
MS6_RS15935 442853..443131 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
MS6_RS15940 443128..443412 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
MS6_RS15945 443606..444121 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
MS6_RS15950 444305..444718 + 414 WP_000049420.1 VOC family protein -
MS6_RS15960 445273..446450 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island qnrVC4 - 297729..445581 147852
- inside Integron qnrVC4 - 302809..445205 142396
flank IS/Tn - - 438153..438590 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T30651 WP_000578476.1 NZ_AP014525:c443131-442853 [Vibrio cholerae MS6]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T30651 NZ_AP014525:c443131-442853 [Vibrio cholerae MS6]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT30651 WP_000381183.1 NZ_AP014525:c443412-443128 [Vibrio cholerae MS6]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT30651 NZ_AP014525:c443412-443128 [Vibrio cholerae MS6]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9K2M8

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References