Detailed information of TA system TA058428

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 424945..425900
• Replicon: chromosome
• Accession: NZ_CP013320
• Organism: Vibrio cholerae strain NCTC9420

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: ASZ86_RS16745
• Protein ID: WP_000118351.1
• Coordinates: 425367..425900 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: ASZ86_RS16740
• Protein ID: WP_001882332.1
• Coordinates: 424945..425370 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ASZ86_RS16690	420475..420726	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ86_RS16695	420716..421003	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ86_RS16700	421131..421586	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
ASZ86_RS16710	421908..422060	+	153	WP_001884520.1	DUF645 family protein	-
ASZ86_RS16715	422262..422759	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
ASZ86_RS16720	422756..423028	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
ASZ86_RS16725	423259..423927	+	669	WP_000043871.1	hypothetical protein	-
ASZ86_RS16730	424079..424366	+	288	WP_000426470.1	hypothetical protein	-
ASZ86_RS16735	424507..424878	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
ASZ86_RS16740	424945..425370	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
ASZ86_RS16745	425367..425900	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
ASZ86_RS16750	426102..426359	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
ASZ86_RS16755	426347..426649	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
ASZ86_RS16760	426883..427800	+	918	WP_000186333.1	alpha/beta hydrolase	-
ASZ86_RS16770	427957..428346	+	390	WP_001081302.1	hypothetical protein	-
ASZ86_RS16775	428482..428691	+	210	Protein_467	GNAT family N-acetyltransferase	-
ASZ86_RS16780	428810..429247	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
ASZ86_RS16785	429311..429529	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
ASZ86_RS16795	429704..430576	+	873	WP_000254716.1	DUF3800 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	304702..436238	131536
	-	inside	Integron	-	-	309782..435862	126080
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	428810..429247	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T58428 WP_000118351.1 NZ_CP013320:425367-425900 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T58428 NZ_CP013320:425367-425900 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT58428 WP_001882332.1 NZ_CP013320:424945-425370 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT58428 NZ_CP013320:424945-425370 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94