Detailed information of TA system TA023736

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 452699..453654
• Replicon: chromosome II
• Accession: NC_012583
• Organism: Vibrio cholerae O395

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: VC395_RS16630
• Protein ID: WP_000118351.1
• Coordinates: 453121..453654 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VC395_RS21085
• Protein ID: WP_001882332.1
• Coordinates: 452699..453124 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VC395_RS16595	449225..449719	-	495	WP_000477435.1	hypothetical protein	-
VC395_RS16600	450016..450513	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
VC395_RS16605	450510..450782	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
VC395_RS16610	451013..451681	+	669	WP_000043871.1	hypothetical protein	-
VC395_RS16615	451833..452120	+	288	WP_000426470.1	hypothetical protein	-
VC395_RS16620	452261..452632	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
VC395_RS21085	452699..453124	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
VC395_RS16630	453121..453654	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
VC395_RS16635	453856..454113	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VC395_RS16640	454101..454403	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VC395_RS16645	454637..455554	+	918	WP_000186333.1	alpha/beta hydrolase	-
VC395_RS16650	455762..456004	+	243	WP_000107462.1	hypothetical protein	-
VC395_RS16655	456179..456568	+	390	WP_001081302.1	hypothetical protein	-
VC395_RS16660	456704..456913	+	210	Protein_484	GNAT family N-acetyltransferase	-
VC395_RS16665	457032..457469	+	438	WP_000503164.1	IS200/IS605-like element IS1004 family transposase	-
VC395_RS16670	457533..457751	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	427343..471178	43835
	-	inside	Integron	-	-	450510..464084	13574
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0.002	flank	IS/Tn	-	-	457032..457469	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T23736 WP_000118351.1 NC_012583:453121-453654 [Vibrio cholerae O395]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T23736 NC_012583:453121-453654 [Vibrio cholerae O395]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT23736 WP_001882332.1 NC_012583:452699-453124 [Vibrio cholerae O395]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT23736 NC_012583:452699-453124 [Vibrio cholerae O395]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94