Detailed information of TA system TA023717

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 400164..401119
• Replicon: chromosome II
• Accession: NC_012580
• Organism: Vibrio cholerae M66-2

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: VCM66_RS15380
• Protein ID: WP_000118351.1
• Coordinates: 400586..401119 (+)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: VCM66_RS19595
• Protein ID: WP_001882332.1
• Coordinates: 400164..400589 (+)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
VCM66_RS15335	395694..395945	+	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCM66_RS15340	395935..396222	+	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCM66_RS15345	396350..396805	+	456	WP_001245327.1	GNAT family N-acetyltransferase	-
VCM66_RS19005	397127..397279	+	153	WP_001884520.1	DUF645 family protein	-
VCM66_RS15350	397481..397978	-	498	WP_000982260.1	GNAT family N-acetyltransferase	-
VCM66_RS15355	397975..398247	-	273	WP_000246253.1	DUF1778 domain-containing protein	-
VCM66_RS15360	398478..399146	+	669	WP_000043871.1	hypothetical protein	-
VCM66_RS15365	399298..399585	+	288	WP_000426470.1	hypothetical protein	-
VCM66_RS15370	399726..400097	+	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
VCM66_RS19595	400164..400589	+	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
VCM66_RS15380	400586..401119	+	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
VCM66_RS15385	401321..401578	+	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
VCM66_RS15390	401566..401868	+	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
VCM66_RS15395	402022..402231	+	210	Protein_419	GNAT family N-acetyltransferase	-
VCM66_RS15400	402350..402787	+	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
VCM66_RS15405	402851..403069	+	219	WP_071908318.1	GNAT family N-acetyltransferase	-
VCM66_RS15410	403244..404116	+	873	WP_000254716.1	DUF3800 domain-containing protein	-
VCM66_RS15415	404266..404865	+	600	WP_001891245.1	glutathione S-transferase N-terminal domain-containing protein	-
VCM66_RS19440	404922..405026	+	105	WP_099607150.1	acetyltransferase	-
VCM66_RS15420	405057..405326	+	270	WP_001114075.1	DUF4160 domain-containing protein	-
VCM66_RS15425	405320..405841	+	522	WP_000921691.1	DUF2442 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	305869..409778	103909
	-	inside	Integron	-	-	310949..409402	98453
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	402350..402787	437

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T23717 WP_000118351.1 NC_012580:400586-401119 [Vibrio cholerae M66-2]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T23717 NC_012580:400586-401119 [Vibrio cholerae M66-2]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT23717 WP_001882332.1 NC_012580:400164-400589 [Vibrio cholerae M66-2]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT23717 NC_012580:400164-400589 [Vibrio cholerae M66-2]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94