Detailed information of TA system TA031953

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /DUF1778(antitoxin)
• Location: 1521700..1522655
• Replicon: chromosome
• Accession: NZ_AP018677
• Organism: Vibrio cholerae strain V060002

Toxin (Protein)

• Gene name: -
• Uniprot ID: Q9KM94
• Locus tag: V060002_RS07360
• Protein ID: WP_000118351.1
• Coordinates: 1521700..1522233 (-)
• Length: 178 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: V060002_RS19480
• Protein ID: WP_001882332.1
• Coordinates: 1522230..1522655 (-)
• Length: 142 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
V060002_RS07315	1517024..1517896	-	873	WP_000254716.1	DUF3800 domain-containing protein	-
V060002_RS07320	1518071..1518289	-	219	WP_071908318.1	GNAT family N-acetyltransferase	-
V060002_RS07325	1518353..1518790	-	438	WP_000503169.1	IS200/IS605-like element IS1004 family transposase	-
V060002_RS07330	1518909..1519118	-	210	Protein_1355	GNAT family N-acetyltransferase	-
V060002_RS07335	1519254..1519643	-	390	WP_001081302.1	hypothetical protein	-
V060002_RS07340	1519800..1520717	-	918	WP_000186333.1	alpha/beta hydrolase	-
V060002_RS07345	1520951..1521253	-	303	WP_000229317.1	type II toxin-antitoxin system RelE/ParE family toxin	-
V060002_RS07350	1521241..1521498	-	258	WP_000861987.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
V060002_RS07360	1521700..1522233	-	534	WP_000118351.1	GNAT family N-acetyltransferase	Toxin
V060002_RS19480	1522230..1522655	-	426	WP_001882332.1	DUF1778 domain-containing protein	Antitoxin
V060002_RS07370	1522722..1523093	-	372	WP_001164080.1	nucleotide pyrophosphohydrolase	-
V060002_RS07375	1523234..1523521	-	288	WP_000426470.1	hypothetical protein	-
V060002_RS07380	1523673..1524341	-	669	WP_000043871.1	hypothetical protein	-
V060002_RS07385	1524572..1524844	+	273	WP_000246253.1	DUF1778 domain-containing protein	-
V060002_RS07390	1524841..1525338	+	498	WP_000982260.1	GNAT family N-acetyltransferase	-
V060002_RS07400	1525540..1525692	-	153	WP_001884520.1	DUF645 family protein	-
V060002_RS07405	1526014..1526469	-	456	WP_001245327.1	GNAT family N-acetyltransferase	-
V060002_RS07410	1526597..1526884	-	288	WP_000221354.1	type II toxin-antitoxin system RelE/ParE family toxin	-
V060002_RS07415	1526874..1527125	-	252	WP_001250179.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	catB9	-	1516275..1622843	106568
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_002506 Vibrio cholerae O1 biovar El Tor str. N16961 IS/Tn TA000179, TA000177, TA000178, TA010035 428335..428772 0	flank	IS/Tn	-	-	1518353..1518790	437
	-	inside	Integron	-	-	1511738..1563819	52081

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (97-153)

Antitoxin

• Vibrio phage ICP1, Orf50 (57-133)

Sequences

Toxin

Protein

• Length: 178 a.a.
• Molecular weight: 20171.46 Da
• Isoelectric Point: 9.1432

>T31953 WP_000118351.1 NZ_AP018677:c1522233-1521700 [Vibrio cholerae]
VSWGKEFVELSKSKHDRDSFDCGEQELNTFIKTQAAKHMQAGISRTMVLPSAHPLLNQKFAICAFYSVAPSSISRETLPA
QLAKKLPRYPIPVFLLAQLAVHKEFHGSGLGKVSLIRALKYLWEVNHHMRAYAIVVDCLTDSAQAFYTKFGFEVLCEHNE
RIRMFLPMKTVEQLFNQ

Nucleotide

• Length: 534 bp

>T31953 NZ_AP018677:c1522233-1521700 [Vibrio cholerae]
GTGAGTTGGGGCAAAGAGTTTGTAGAACTTAGTAAATCAAAGCATGACCGCGACTCATTTGACTGTGGTGAGCAAGAGCT
AAACACATTTATCAAAACTCAAGCTGCAAAGCACATGCAAGCAGGTATTAGCCGAACAATGGTTTTACCTAGTGCTCACC
CACTGTTAAATCAAAAATTCGCTATTTGTGCCTTTTATAGTGTTGCACCGAGCTCAATCAGCCGAGAAACATTACCGGCA
CAATTAGCAAAGAAACTACCGAGGTATCCAATCCCTGTTTTTCTGTTGGCTCAGTTAGCTGTGCACAAAGAATTTCACGG
TTCTGGACTTGGAAAGGTCAGTCTAATCCGTGCGCTGAAGTACTTGTGGGAAGTGAATCATCACATGAGAGCATACGCAA
TAGTTGTGGATTGTCTGACAGATTCCGCTCAAGCGTTCTATACGAAGTTCGGCTTTGAAGTATTGTGCGAACATAATGAG
CGTATTCGTATGTTCTTACCGATGAAAACGGTAGAACAACTTTTTAATCAATAG

Antitoxin

Protein

• Length: 142 a.a.
• Molecular weight: 15714.41 Da
• Isoelectric Point: 7.6659

>AT31953 WP_001882332.1 NZ_AP018677:c1522655-1522230 [Vibrio cholerae]
MLCLSLVVMRCQPLRRALYSPSPRKLSVRIECALSLAYNRSTDGMYPYAGGVMATARLDIRLDEEIKAKAEKASALLGLK
SLTEYVVRLMDEDATHVIEEHESIMVKDSVFDEFMAACNKVKAPNQALLEAVKFTEKSGIK

Nucleotide

• Length: 426 bp

>AT31953 NZ_AP018677:c1522655-1522230 [Vibrio cholerae]
TTGTTGTGTTTGAGTTTAGTGGTCATGCGTTGTCAGCCCCTTAGACGGGCGTTATATTCTCCCTCGCCCCGTAAACTTTC
CGTACGAATTGAATGTGCTTTAAGTTTGGCCTATAATCGCTCAACTGACGGAATGTATCCGTACGCAGGAGGGGTTATGG
CAACGGCACGTTTAGATATCCGCCTTGATGAAGAGATCAAGGCAAAAGCTGAAAAGGCATCGGCTTTACTTGGTTTAAAA
AGTCTCACTGAATATGTGGTGAGACTTATGGACGAAGATGCGACTCATGTTATTGAGGAGCATGAAAGTATTATGGTTAA
AGACAGTGTTTTTGACGAGTTTATGGCTGCGTGTAATAAAGTAAAAGCACCGAATCAAGCTTTGCTTGAAGCTGTTAAAT
TTACAGAGAAGAGCGGTATCAAGTGA

Structures

Toxin

• AlphaFold DB: Q9KM94