58256

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-RelB
Location 424946..425505 Replicon chromosome
Accession NZ_CP013304
Organism Vibrio cholerae strain CRC711

Toxin (Protein)


Gene name relE Uniprot ID Q9K2M8
Locus tag ASZ82_RS16715 Protein ID WP_000578476.1
Coordinates 424946..425224 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID Q9L991
Locus tag ASZ82_RS16720 Protein ID WP_000381183.1
Coordinates 425221..425505 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ASZ82_RS16665 420246..420683 + 438 WP_000503169.1 IS200/IS605-like element IS1004 family transposase -
ASZ82_RS16670 420747..420965 + 219 WP_071908318.1 GNAT family N-acetyltransferase -
ASZ82_RS16680 421140..422012 + 873 WP_000254716.1 DUF3800 domain-containing protein -
ASZ82_RS16685 422162..422761 + 600 WP_001891245.1 glutathione S-transferase N-terminal domain-containing protein -
ASZ82_RS20195 422818..422922 + 105 WP_099607150.1 acetyltransferase -
ASZ82_RS16690 422953..423222 + 270 WP_001114075.1 DUF4160 domain-containing protein -
ASZ82_RS16695 423216..423737 + 522 WP_000921691.1 DUF2442 domain-containing protein -
ASZ82_RS20200 424036..424161 + 126 WP_001905700.1 DUF645 family protein -
ASZ82_RS16710 424412..424807 + 396 WP_001000867.1 hypothetical protein -
ASZ82_RS16715 424946..425224 - 279 WP_000578476.1 type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family Toxin
ASZ82_RS16720 425221..425505 - 285 WP_000381183.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
ASZ82_RS16725 425699..426214 + 516 WP_000343779.1 GNAT family N-acetyltransferase -
ASZ82_RS16730 426398..426811 + 414 WP_000049420.1 VOC family protein -
ASZ82_RS16745 427366..428543 + 1178 WP_086010879.1 IS3-like element ISVch4 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island catB9 - 304736..427674 122938
- inside Integron - - 309816..427298 117482
flank IS/Tn - - 420246..420683 437


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(2-89)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(5-70)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10819.59 Da        Isoelectric Point: 5.1651

>T58256 WP_000578476.1 NZ_CP013304:c425224-424946 [Vibrio cholerae]
MIFWEEASLNDREKIFEFLYDFNPAAAKKTDELIEAKVENLLEQPLIGVQRDGIRGRLLIIPEISMIVSYWVDGSKIRIM
RVLHQKQKFPND

Download         Length: 279 bp

>T58256 NZ_CP013304:c425224-424946 [Vibrio cholerae]
ATGATTTTCTGGGAAGAAGCATCTCTCAATGATCGTGAGAAAATTTTCGAATTTCTCTACGACTTTAACCCAGCAGCGGC
TAAAAAAACGGATGAGCTCATAGAAGCCAAAGTCGAAAATTTGCTTGAGCAACCACTAATCGGTGTTCAGCGTGATGGCA
TTAGAGGCAGATTGCTTATTATCCCTGAGATATCAATGATTGTTTCGTATTGGGTTGATGGTTCTAAAATTCGAATAATG
CGTGTACTACATCAGAAACAAAAATTTCCAAACGACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10901.25 Da        Isoelectric Point: 8.0775

>AT58256 WP_000381183.1 NZ_CP013304:c425505-425221 [Vibrio cholerae]
MDTRIQFRVDEETKRLAQQMAESQGRTLSDACRELTEQLAEQQRKALSHDAWLTEQVNQAFEKFDSGKAVFIEHDIAKAR
MAERKAKIRNRGHA

Download         Length: 285 bp

>AT58256 NZ_CP013304:c425505-425221 [Vibrio cholerae]
ATGGATACTAGAATTCAATTTCGTGTAGACGAAGAAACAAAACGTTTAGCTCAACAAATGGCTGAAAGCCAAGGTCGAAC
TCTTAGCGATGCTTGCCGTGAACTCACTGAACAATTAGCTGAGCAGCAACGCAAGGCATTATCTCACGATGCATGGCTAA
CTGAACAAGTGAATCAAGCATTTGAGAAGTTCGACTCAGGAAAAGCAGTATTCATTGAACATGACATCGCCAAAGCACGA
ATGGCTGAACGTAAAGCTAAAATCCGAAATCGAGGCCACGCATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9K2M8

Antitoxin

Source ID Structure
AlphaFold DB A0A067BL33

References