Detailed information of TA system TA010041

Experimentally validated

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/-
• Location: 1024211..1025184
• Replicon: chromosome
• Accession: NC_000962
• Organism: Mycobacterium tuberculosis H37Rv

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: I6XA42
• Locus tag: Rv0919
• Protein ID: NP_215434.1
• Coordinates: 1024684..1025184 (+)
• Length: 167 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: O05910
• Locus tag: Rv0918
• Protein ID: NP_215433.1
• Coordinates: 1024211..1024687 (+)
• Length: 159 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
Rv0915c	1020058..1021329	-	1272	YP_177765.1	PPE family protein PPE14	-
Rv0916c	1021344..1021643	-	300	YP_177766.1	PE family protein PE7	-
Rv0917	1022087..1023868	+	1782	NP_215432.1	glycine betaine transport integral membrane protein BetP	-
Rv0918	1024211..1024687	+	477	NP_215433.1	hypothetical protein	Antitoxin
Rv0919	1024684..1025184	+	501	NP_215434.1	GCN5-like N-acetyltransferase	Toxin
Rv0920c	1025497..1026816	-	1320	NP_215435.1	transposase	-
Rv0921	1027104..1027685	+	582	NP_215436.1	resolvase	-
Rv0922	1027685..1029337	+	1653	NP_215437.1	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NZ_CP018302 Mycobacterium tuberculosis strain I0004000-1 Prophage TA069443, TA069444 1011497..1071224 0.008 View NZ_CP039851 Mycobacterium tuberculosis variant bovis strain Danish delta-sapM Prophage TA124457, TA124458 1010968..1070700 0.008 View NZ_CP041803 Mycobacterium tuberculosis strain SEA11278P6C4 Prophage TA130171, TA130172 1012828..1072558 0.008 View NZ_CP041807 Mycobacterium tuberculosis strain SEA11020038P6C4 Prophage TA130413, TA130414 1013154..1072885 0.008 View NZ_CP041866 Mycobacterium tuberculosis strain 1-0036P6C4 Prophage TA133972, TA133973 1012291..1072022 0.008	inside	Prophage	-	-	1023865..1069776	45911

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (38-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (74-150)

Sequences

Toxin

Protein

• Length: 167 a.a.
• Molecular weight: 18048.55 Da
• Isoelectric Point: 7.8659

>T10041 NP_215434.1 NC_000962:1024684-1025184 [Mycobacterium tuberculosis H37Rv]
VSGYSAPRRISDADDVTSFSSGEPSLDDYLRKRALANHVQGGSRCFVTCRDGRVVGFYALASGSVAHADAPGRVRRNMPD
PVPVILLSRLAVDRKEQGRGLGSHLLRDAIGRCVQAADSIGLRAILVHALHDEARAFYVHFDFEISPTDPLHLMLLMKDA
RALIGD

Nucleotide

• Length: 501 bp

>T10041 NC_000962:1024684-1025184 [Mycobacterium tuberculosis H37Rv]
GTGAGCGGCTACAGCGCGCCGCGACGTATCAGCGACGCCGATGACGTCACGAGCTTCAGCAGCGGCGAGCCCAGTCTGGA
CGATTACTTGCGCAAGCGGGCGTTGGCCAACCATGTGCAGGGAGGGTCGCGCTGTTTCGTGACGTGCCGTGACGGTCGGG
TAGTCGGCTTCTATGCGCTAGCGTCAGGGTCGGTCGCACACGCTGATGCTCCGGGACGGGTGCGCCGCAATATGCCTGAC
CCCGTGCCGGTGATCCTGCTGTCGCGGTTGGCGGTTGATCGCAAAGAACAGGGCAGGGGCCTGGGCAGTCATCTGCTGCG
TGATGCGATCGGTCGCTGTGTCCAGGCTGCGGACTCGATCGGGCTGCGGGCGATTCTTGTTCATGCGTTGCACGATGAGG
CCCGCGCGTTCTACGTCCACTTTGACTTCGAGATCTCGCCGACCGATCCGCTGCACCTAATGCTGTTGATGAAAGACGCT
CGCGCGCTAATTGGCGACTGA

Antitoxin

Protein

• Length: 159 a.a.
• Molecular weight: 17387.98 Da
• Isoelectric Point: 11.2467

>AT10041 NP_215433.1 NC_000962:1024211-1024687 [Mycobacterium tuberculosis H37Rv]
MHRAGAAVTANVWCRAGGIRMAPRPVIPVATQQRLRRQADRQSLGSSGLPALNCTPIRHTIDVMATKPERKTERLAARLT
PEQDALIRRAAEAEGTDLTNFTVTAALAHARDVLADRRLFVLTDAAWTEFLAALDRPVSHKPRLEKLFAARSIFDTEG

Nucleotide

• Length: 477 bp

>AT10041 NC_000962:1024211-1024687 [Mycobacterium tuberculosis H37Rv]
TTGCACCGAGCGGGAGCGGCGGTGACGGCCAACGTGTGGTGTCGTGCGGGCGGCATTCGGATGGCGCCACGGCCGGTCAT
CCCGGTGGCTACGCAGCAGCGCCTGCGGCGGCAGGCGGATCGCCAGAGCCTGGGTAGTAGCGGCTTGCCAGCGTTGAATT
GTACGCCTATCAGGCACACAATTGATGTCATGGCTACCAAGCCTGAGCGGAAGACCGAGCGTCTTGCAGCGCGCCTGACC
CCTGAGCAGGACGCGCTGATTCGTCGTGCTGCCGAGGCCGAGGGGACTGACCTCACCAATTTCACGGTTACAGCGGCGTT
GGCGCACGCGCGCGACGTGCTGGCCGACCGCCGGCTCTTCGTACTCACCGATGCCGCGTGGACTGAGTTCCTCGCCGCGC
TGGACCGGCCCGTCTCACACAAGCCTCGGTTGGAGAAGCTGTTCGCCGCGCGGTCCATTTTCGACACCGAGGGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Toxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
T443	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	50.327	95.031	0.478
T10051	Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023	46.795	95.706	0.448
T199	Escherichia coli O157:H7 str. EDL933	39.61	95.652	0.379
T10052	Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023	33.121	94.578	0.313
T177	Vibrio cholerae O1 biovar El Tor str. N16961	30.675	98.193	0.301

Multiple sequence alignment

Antitoxin

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
AT199	Escherichia coli O157:H7 str. EDL933	40.789	80.851	0.33
AT443	Salmonella enterica subsp. enterica serovar Typhimurium str. LT2	37.349	87.368	0.326

Multiple sequence alignment

Structures

Toxin

• AlphaFold DB: I6XA42

Antitoxin

• AlphaFold DB: O05910

References

(1) Francesca G Tomasi et al. (2022) A tRNA-Acetylating Toxin and Detoxifying Enzyme in Mycobacterium tuberculosis. Microbiology Spectrum 10(3):e0058022. [PubMed:35638832]


 experimental literature