Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) unclassified/Polyketide_cyc2(toxin)
Location 1016132..1016751 Replicon chromosome
Accession NZ_CP018302
Organism Mycobacterium tuberculosis strain I0004000-1

Toxin (Protein)


Gene name novel[antitoxin] Uniprot ID -
Locus tag BOJ12_RS04955 Protein ID WP_003404726.1
Coordinates 1016317..1016751 (+) Length 145 a.a.

Antitoxin (Protein)


Gene name novel[toxin] Uniprot ID P9WJ06
Locus tag BOJ12_RS04950 Protein ID WP_003898641.1
Coordinates 1016132..1016311 (+) Length 60 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BOJ12_RS04940 1011605..1013185 + 1581 WP_003909313.1 serine hydrolase -
BOJ12_RS04945 1013182..1015575 + 2394 WP_003898639.1 cation-translocating P-type ATPase -
BOJ12_RS21830 1015848..1016006 + 159 WP_003404720.1 hypothetical protein -
BOJ12_RS04950 1016132..1016311 + 180 WP_003898641.1 antitoxin Antitoxin
BOJ12_RS04955 1016317..1016751 + 435 WP_003404726.1 SRPBCC family protein Toxin
BOJ12_RS04960 1016849..1017622 + 774 WP_003404735.1 VOC family protein -
BOJ12_RS04965 1017687..1018136 + 450 WP_003404738.1 hypothetical protein -
BOJ12_RS04970 1018271..1018501 - 231 WP_003898642.1 hypothetical protein -
BOJ12_RS04975 1018668..1020176 - 1509 WP_003404742.1 carotenoid oxygenase family protein -
BOJ12_RS04980 1020178..1021416 - 1239 WP_003404744.1 acetyl-CoA acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 1011497..1071224 59727


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Polyketide cyclase/dehydrase

(1-142)

Antitoxin

Antitoxin Rv0909/MT0933

(4-50)


Sequences

Toxin        


Download         Length: 145 a.a.        Molecular weight: 15754.47 Da        Isoelectric Point: 9.7977

>T69443 WP_003404726.1 NZ_CP018302:1016317-1016751 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Download         Length: 435 bp

>T69443 NZ_CP018302:1016317-1016751 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin


Download         Length: 60 a.a.        Molecular weight: 6403.07 Da        Isoelectric Point: 4.5393

>AT69443 WP_003898641.1 NZ_CP018302:1016132-1016311 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Download         Length: 180 bp

>AT69443 NZ_CP018302:1016132-1016311 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
AlphaFold DB A0A045KDK5

References