10052

Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) tacAT/-
Location 4599903..4600708 Replicon chromosome
Accession UFRD01000002
Organism Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023

Toxin (Protein)


Gene name TacT3 Uniprot ID -
Locus tag NCTC12023_04871 Protein ID SUI00082.1
Coordinates 4599903..4600430 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name TacA3 Uniprot ID -
Locus tag NCTC12023_04872 Protein ID SUI00083.1
Coordinates 4600427..4600708 (-) Length 94 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
NCTC12023_04868 4595203..4597770 - 2568 SUI00079.1 DNA mismatch repair protein MutS -
NCTC12023_04869 4598622..4599278 - 657 SUI00080.1 serine/threonine protein phosphatase -
NCTC12023_04870 4599654..4599830 + 177 SUI00081.1 putative cytoplasmic protein -
NCTC12023_04871 4599903..4600430 - 528 SUI00082.1 acetyltransferase Toxin
NCTC12023_04872 4600427..4600708 - 282 SUI00083.1 ABC transporter Antitoxin
NCTC12023_04873 4601406..4601732 + 327 SUI00084.1 putative cytoplasmic protein -
NCTC12023_04874 4602005..4602337 - 333 SUI00085.1 PrpA and PrpB, metallophosphatase domain protein -
NCTC12023_04875 4602337..4602786 - 450 SUI00086.1 membrane protein -
NCTC12023_04877 4603217..4603660 - 444 SUI00087.1 cell adherance/invasion protein -
NCTC12023_04878 4604018..4604767 + 750 SUI00088.1 invasion protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - invH / invF / invG / invE / invA / invB 4599654..4610080 10426
flank IS/Tn - - 4599654..4599830 176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

(4-92)


Sequences


Toxin        


Download         Length: 176 a.a.        Molecular weight: 19069.91 Da        Isoelectric Point: 9.6420

>T10052 SUI00082.1 UFRD01000002:c4600430-4599903 [Salmonella enterica subsp. enterica serovar Typhimurium]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHARQNHEKGTTKTYVALDNSDVTRIHGFYSVSPASLIYAQVPGAISK
GLGRYDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQVCDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Download         Length: 528 bp

>T10052 UFRD01000002:c4600430-4599903 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10318.10 Da        Isoelectric Point: 6.3133

>AT10052 SUI00083.1 UFRD01000002:c4600708-4600427 [Salmonella enterica subsp. enterica serovar Typhimurium]
MPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNILPVAQKIVDAAERVYLTERDTKMIMEILDNPPAPNEK
LLAAAFALPDMKK

Download         Length: 282 bp

>AT10052 UFRD01000002:c4600708-4600427 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGATAGTCCGCGC
TGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTGTTGATGCCG
CCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCGAATGAAAAG
CTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T199 Escherichia coli O157:H7 str. EDL933

35.172

90.062

0.317

T10041 Mycobacterium tuberculosis H37Rv

33.121

94.578

0.313


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value
AT200 Escherichia coli O157:H7 str. EDL933

35.366

93.182

0.33

AT10120 Klebsiella pneumoniae subsp. pneumoniae HS11286

36.709

89.773

0.33


Multiple sequence alignment    




Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure

References


(1) Julian A Rycroft et al. (2018) Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection. Nature Communications 9(1):1993. [PubMed:29777131] experimental

(2) Grzegorz J Grabe et al. (2021) Auxiliary interfaces support the evolution of specific toxin-antitoxin pairing. Nature Chemical Biology 17(12):1296-1304. [PubMed:34556858] 3D_structure


experimental experimental literature
3D_structure protein structure literature