Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 996063..996877 Replicon chromosome
Accession NZ_AP023294
Organism Salmonella enterica subsp. enterica serovar 4[5]12:i:- strain L-4261

Toxin (Protein)


Gene name TacT3 Uniprot ID Q57KM2
Locus tag K5635_RS04745 Protein ID WP_000971655.1
Coordinates 996063..996590 (-) Length 176 a.a.

Antitoxin (Protein)


Gene name TacA3 Uniprot ID E8XL32
Locus tag K5635_RS04750 Protein ID WP_000855692.1
Coordinates 996587..996877 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
K5635_RS04725 (991363) 991363..993930 - 2568 WP_001005807.1 DNA mismatch repair protein MutS -
K5635_RS04730 (994089) 994089..994610 + 522 WP_000858988.1 hypothetical protein -
K5635_RS04735 (994782) 994782..995438 - 657 WP_000420452.1 protein-serine/threonine phosphatase -
K5635_RS04740 (995785) 995785..995990 + 206 Protein_931 IS5/IS1182 family transposase -
K5635_RS04745 (996063) 996063..996590 - 528 WP_000971655.1 GNAT family N-acetyltransferase Toxin
K5635_RS04750 (996587) 996587..996877 - 291 WP_000855692.1 DUF1778 domain-containing protein Antitoxin
K5635_RS04755 (997147) 997147..997325 - 179 Protein_934 IS3 family transposase -
K5635_RS04760 (997566) 997566..997892 + 327 WP_000393302.1 hypothetical protein -
K5635_RS04765 (998165) 998165..998512 - 348 WP_001669174.1 DUF1493 family protein -
K5635_RS04770 (998497) 998497..998946 - 450 WP_000381610.1 membrane protein -
K5635_RS04775 (999377) 999377..999820 - 444 WP_000715092.1 SPI-1 type III secretion system invasion lipoprotein InvH -
K5635_RS04780 (1000277) 1000277..1000927 + 651 WP_001674874.1 type III secretion system transcriptional activator InvF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - invH / invF / invG / invE / invA / invB 995814..1006240 10426
flank IS/Tn - - 995814..995990 176


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Vibrio phage ICP1, Orf50

(1-95)


Sequences

Toxin        


Download         Length: 176 a.a.        Molecular weight: 19069.91 Da        Isoelectric Point: 9.6420

>T37294 WP_000971655.1 NZ_AP023294:c996590-996063 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MMFTDWHEAAIGKTHNRMNFDCGDADLNQFLQRHARQNHEKGTTKTYVALDNSDVTRIHGFYSVSPASLIYAQVPGAISK
GLGRYDVPVFRLGRLAVDKSMQGQGLGAQLLLSAGKRCIQAALQVGGVALLIDAKNKQVCDWYKGFGAVPLNDQPLSLLL
SFKTLYAALSASGRL

Download         Length: 528 bp

>T37294 NZ_AP023294:c996590-996063 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTGCAACGCCATGCACGACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATACGATGTGCCGGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGTGGCGTAGCCCTACTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACGATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10678.57 Da        Isoelectric Point: 8.5779

>AT37294 WP_000855692.1 NZ_AP023294:c996877-996587 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
MKTMPQIAIESNERLSLRVSTDAKKLIVRAAAIQQTNLTDFVVSNILPVAQKIVDAAERVYLTERDTKMIMEILDNPPAP
NEKLLAAAFALPDMKK

Download         Length: 291 bp

>AT37294 NZ_AP023294:c996877-996587 [Salmonella enterica subsp. enterica serovar 4,[5],12:i:-]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGGCTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACATACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGCGATACTAAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6G96
PDB 7AK9

Antitoxin

Source ID Structure
PDB 7AK9
AlphaFold DB A0A625WHV3

References