Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ataRT/RHH-GNAT |
| Location | 4384073..4384873 | Replicon | chromosome |
| Accession | NC_002655 | ||
| Organism | Escherichia coli O157:H7 str. EDL933 | ||
Toxin (Protein)
| Gene name | ataT | Uniprot ID | Q8XED1 |
| Locus tag | Z_RS22685 | Protein ID | WP_000342450.1 |
| Coordinates | 4384073..4384600 (-) | Length | 176 a.a. |
Antitoxin (Protein)
| Gene name | ataR | Uniprot ID | F4NNI1 |
| Locus tag | Z_RS22690 | Protein ID | WP_001277108.1 |
| Coordinates | 4384607..4384873 (-) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Z_RS22660 (Z4825) | 4379148..4379915 | - | 768 | WP_000082101.1 | high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG | - |
| Z_RS22665 (Z4826) | 4379912..4381189 | - | 1278 | WP_000803797.1 | branched chain amino acid ABC transporter permease LivM | - |
| Z_RS22670 (Z4827) | 4381186..4382112 | - | 927 | WP_001295111.1 | high-affinity branched-chain amino acid ABC transporter permease LivH | - |
| Z_RS22675 (Z4829) | 4382160..4383269 | - | 1110 | WP_001301528.1 | high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK | - |
| Z_RS22680 (Z4831) | 4383693..4384076 | + | 384 | WP_000778769.1 | aspartate 1-decarboxylase autocleavage activator PanM | - |
| Z_RS22685 (Z4832) | 4384073..4384600 | - | 528 | WP_000342450.1 | GNAT family N-acetyltransferase | Toxin |
| Z_RS22690 (Z4833) | 4384607..4384873 | - | 267 | WP_001277108.1 | DUF1778 domain-containing protein | Antitoxin |
| Z_RS22695 (Z4834) | 4385023..4386126 | - | 1104 | WP_001021996.1 | branched chain amino acid ABC transporter substrate-binding protein LivJ | - |
| Z_RS22700 (Z4835) | 4386397..4387251 | - | 855 | WP_000130217.1 | RNA polymerase sigma factor RpoH | - |
| Z_RS22705 (Z4836) | 4387496..4388554 | - | 1059 | WP_001042018.1 | permease-like cell division protein FtsX | - |
| Z_RS22710 (Z4837) | 4388547..4389215 | - | 669 | WP_000617723.1 | cell division ATP-binding protein FtsE | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 176 a.a. Molecular weight: 19678.61 Da Isoelectric Point: 6.9489
>T200 WP_000342450.1 NC_002655:c4384600-4384073 [Escherichia coli O157:H7 str. EDL933]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMNVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLCGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMNVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD
Download Length: 528 bp
>T200 NC_002655:c4384600-4384073 [Escherichia coli O157:H7 str. EDL933]
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAACGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA
ATGGATGATCTGACGATAGAGATTCTGACCGATGATGCAGATTATGATCTACAGCGATTCGACTGCGGCGAGGAAGCGTT
AAATCTCTTTCTGACGACACATCTCGTTCGTCAACATCGCAACAAAATTCTGCGAGCGTATATCCTTTGTCGCAACACTC
CAGAACGTCAGGTGCTGGGATATTACACATTATGCGGCAGTTGTTTTGAACGAGCCGCATTGCCCTCGAAATCGAAACAG
AAAAAAATTCCCTACAAAAATATTCCCAGCGTTACTCTTGGGCGTCTGGCAATTGATCGTTCATTACAGGGGCAGGGATG
GGGAGCAACACTGGTTGCTCATGCCATGAACGTCGTCTGGTCAGCCTCTTTAGCGGTAGGTATTCACGGTCTTTTTGTCG
AGGCGCTGAATGAAAAAGCCCATACGTTTTATAAATCGCTGGGCTTTATCCCTTTAGTCGGGGAAAACGAAAATGCGTTA
TTTTTCCCAACCAAATCCATTGAACTGCTTTTTACACAGAGCGATTAA
Antitoxin
Download Length: 89 a.a. Molecular weight: 9910.31 Da Isoelectric Point: 10.0863
>AT200 WP_001277108.1 NC_002655:c4384873-4384607 [Escherichia coli O157:H7 str. EDL933]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWTRVMDALSNPPSPGEKLKR
AAKRLQGM
Download Length: 267 bp
>AT200 NC_002655:c4384873-4384607 [Escherichia coli O157:H7 str. EDL933]
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA
ATGTCTGCTGTTAAAAAGCAGCGTATCGATCTGCGTTTAACTGACGATGACAAAAGTATGATTGAGGAAGCGGCAGCGAT
ATCTAATCAGTCCGTCAGCCAATTTATGTTGAACAGCGCTTCGCAGCGGGCTGCGGAAGTGATTGAACAGCATCGGCGGG
TGATCCTCAATGAGGAATCCTGGACGCGGGTGATGGATGCGCTGAGTAATCCACCGTCACCAGGTGAAAAGCTAAAACGT
GCGGCAAAACGTCTTCAGGGAATGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T10120 | Klebsiella pneumoniae subsp. pneumoniae HS11286 |
67.442 |
98.286 |
0.663 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10120 | Klebsiella pneumoniae subsp. pneumoniae HS11286 |
73.256 |
97.727 |
0.716 |
| AT10052 | Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023 |
35.366 |
93.182 |
0.33 |
| AT176 | Vibrio cholerae O1 biovar El Tor str. N16961 |
33.333 |
92.045 |
0.307 |
| AT165 | Vibrio cholerae O1 biovar El Tor str. N16961 |
33.333 |
92.045 |
0.307 |
Multiple sequence alignment
References
(1) Dukas Jurėnas et al. (2017) AtaT blocks translation initiation by N-acetylation of the initiator tRNAfMet. Nature Chemical Biology 13(6):640-646. [PubMed:28369041]
(2) Yuka Yashiro et al. (2019) Crystal Structure of the Enterohemorrhagic Escherichia coli AtaT-AtaR Toxin-Antitoxin Complex. Structure (London, England : 1993) 27(3):476-484.e3. [PubMed:30612860]
(3) Laurence Van Melderen et al. (2018) Messing up translation from the start: How AtaT inhibits translation initiation in E. coli. RNA Biology 15(3):303-307. [PubMed:29099338]
(4) Dukas Jurėnas et al. (2019) Mechanism of regulation and neutralization of the AtaR-AtaT toxin-antitoxin system. Nature Chemical Biology 15(3):285-294. [PubMed:30718814]
(5) Dukas Jurėnas et al. (2018) Crystallization and X-ray analysis of all of the players in the autoregulation of the ataRT toxin-antitoxin system. Acta Crystallographica. Section F, Structural Biology Communications 74(Pt 7):391-401. [PubMed:29969102]
(6) Zhili He et al. (2021) AtaT Improves the Stability of Pore-Forming Protein EspB by Acetylating Lysine 206 to Enhance Strain Virulence. Frontiers in Microbiology 12:627141. [PubMed:33732222]
(7) Yuka Yashiro et al. (2020) Mechanism of aminoacyl-tRNA acetylation by an aminoacyl-tRNA acetyltransferase AtaT from enterohemorrhagic E. coli. Nature Communications 11(1):5438. [PubMed:33116145]