Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | kacAT/- |
| Location | 624695..625505 | Replicon | chromosome |
| Accession | NC_016845 | ||
| Organism | Klebsiella pneumoniae subsp. pneumoniae HS11286 | ||
Toxin (Protein)
| Gene name | KacT | Uniprot ID | A0A0H3GMP0 |
| Locus tag | KPHS_05890 | Protein ID | YP_005224889.1 |
| Coordinates | 624972..625505 (+) | Length | 178 a.a. |
Antitoxin (Protein)
| Gene name | KacA | Uniprot ID | A0A0H3GLZ1 |
| Locus tag | KPHS_05880 | Protein ID | YP_005224888.1 |
| Coordinates | 624695..624961 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KPHS_05830 | 620567..620911 | - | 345 | YP_005224883.1 | copper-binding protein | - |
| KPHS_05840 | 620929..622314 | - | 1386 | YP_005224884.1 | copper/silver efflux system outer membrane protein CusC | - |
| KPHS_05850 | 622281..622421 | - | 141 | YP_005224885.1 | hypothetical protein | - |
| KPHS_05860 | 622486..623169 | + | 684 | YP_005224886.1 | transcriptional activator protein Irlr | - |
| KPHS_05870 | 623159..624592 | + | 1434 | YP_005224887.1 | sensor kinase CusS | - |
| KPHS_05880 | 624695..624961 | + | 267 | YP_005224888.1 | DUF1778 domain-containing protein | Antitoxin |
| KPHS_05890 | 624972..625505 | + | 534 | YP_005224889.1 | putative GCN5-related N-acetyltransferase | Toxin |
| KPHS_05900 | 625553..626674 | - | 1122 | YP_005224890.1 | cupin domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 178 a.a. Molecular weight: 19838.70 Da Isoelectric Point: 5.2614
>T10120 YP_005224889.1 NC_016845:624972-625505 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES
Download Length: 534 bp
>T10120 NC_016845:624972-625505 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGTGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAAGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA
Antitoxin
Download Length: 89 a.a. Molecular weight: 9996.46 Da Isoelectric Point: 10.1757
>AT10120 YP_005224888.1 NC_016845:624695-624961 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR
Download Length: 267 bp
>AT10120 NC_016845:624695-624961 [Klebsiella pneumoniae subsp. pneumoniae HS11286]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T200 | Escherichia coli O157:H7 str. EDL933 |
67.442 |
98.286 |
0.663 |
| T165 | Vibrio cholerae O1 biovar El Tor str. N16961 |
32.716 |
98.182 |
0.321 |
| T176 | Vibrio cholerae O1 biovar El Tor str. N16961 |
32.716 |
98.182 |
0.321 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT200 | Escherichia coli O157:H7 str. EDL933 |
73.256 |
97.727 |
0.716 |
| AT10052 | Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023 |
36.709 |
89.773 |
0.33 |
| AT4502 | Escherichia coli HS |
35.802 |
92.045 |
0.33 |
| AT176 | Vibrio cholerae O1 biovar El Tor str. N16961 |
32.927 |
93.182 |
0.307 |
| AT165 | Vibrio cholerae O1 biovar El Tor str. N16961 |
32.927 |
93.182 |
0.307 |
Multiple sequence alignment
References
(1) Hongliang Qian et al. (2018) Identification and characterization of acetyltransferase-type toxin-antitoxin locus in Klebsiella pneumoniae. Molecular Microbiology 108(4):336-349. [PubMed:29461656]
(2) Hongliang Qian et al. (2019) Toxin-antitoxin operon kacAT of Klebsiella pneumoniae is regulated by conditional cooperativity via a W-shaped KacA-KacT complex. Nucleic Acids Research 47(14):7690-7702. [PubMed:31260525]