Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | itaRT/RHH-GNAT |
| Location | 511857..512742 | Replicon | chromosome |
| Accession | NC_009800 | ||
| Organism | Escherichia coli HS | ||
Toxin (Protein)
| Gene name | itaT | Uniprot ID | - |
| Locus tag | EcHS_A0501 | Protein ID | 157159951 |
| Coordinates | 511857..512399 (-) | Length | 181 a.a. |
Antitoxin (Protein)
| Gene name | itaR | Uniprot ID | - |
| Locus tag | EcHS_A0502 | Protein ID | 157159952 |
| Coordinates | 512383..512694 (-) | Length | 104 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| ECHS_RS02460 (EcHS_A0497) | 507396..508307 | - | 912 | WP_000705874.1 | 2-dehydropantoate 2-reductase | - |
| ECHS_RS02465 (EcHS_A0498) | 508475..508966 | + | 492 | WP_001138904.1 | nucleotide binding protein YajQ | - |
| ECHS_RS02470 (EcHS_A0499) | 509094..510458 | - | 1365 | WP_001000978.1 | MFS transporter | - |
| ECHS_RS02475 (EcHS_A0500) | 510866..511801 | + | 936 | WP_024186686.1 | sel1 repeat family protein | - |
| ECHS_RS02480 (EcHS_A0501) | 511857..512399 | - | 543 | WP_000227784.1 | GNAT family N-acetyltransferase | Toxin |
| ECHS_RS02485 (EcHS_A0502) | 512383..512694 | - | 312 | WP_001297137.1 | DUF1778 domain-containing protein | Antitoxin |
| ECHS_RS02490 (EcHS_A0503) | 512879..513769 | - | 891 | WP_000971336.1 | heme o synthase | - |
| ECHS_RS02495 (EcHS_A0504) | 513781..514110 | - | 330 | WP_000019869.1 | cytochrome o ubiquinol oxidase subunit IV | - |
| ECHS_RS02500 (EcHS_A0505) | 514110..514724 | - | 615 | WP_000179819.1 | cytochrome o ubiquinol oxidase subunit III | - |
| ECHS_RS02505 (EcHS_A0506) | 514714..516705 | - | 1992 | WP_000467180.1 | cytochrome o ubiquinol oxidase subunit I | - |
| ECHS_RS02510 (EcHS_A0507) | 516727..517674 | - | 948 | WP_001239441.1 | cytochrome o ubiquinol oxidase subunit II | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 181 a.a. Molecular weight: 19805.02 Da Isoelectric Point: 8.3395
>T4502 157159951 NC_009800:c512399-511857 [Escherichia coli HS]
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA
MVDKHEEITLPIVLSCNYQSDITYPGQKQFDCGNPVIDKFVRASLKKSVRNSDCAAKALIDRQSGELIGICTFTAYSLEK
QRVSGVLQGSQPSEIGVVRLVMLGVARKYQKRGFGQDLLCDFFEHVKIIHQALPIKGVYLDADPAAINFYARLGFVQLSA
TPNAFGAVPMFLAIQHILAA
Download Length: 543 bp
>T4502 NC_009800:c512399-511857 [Escherichia coli HS]
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG
ATGGTCGACAAGCATGAAGAGATTACTCTGCCCATAGTCCTCTCCTGTAATTATCAGTCTGATATTACTTATCCTGGGCA
AAAACAGTTTGATTGCGGTAACCCTGTTATCGATAAATTTGTACGCGCATCGCTAAAGAAAAGTGTGCGTAATAGCGACT
GTGCGGCTAAAGCACTTATTGACAGACAAAGTGGTGAACTGATCGGCATCTGTACTTTTACGGCATATTCGCTGGAAAAA
CAACGCGTTTCTGGCGTCCTTCAGGGTTCACAACCTTCAGAAATTGGTGTTGTCAGATTAGTCATGTTGGGGGTAGCACG
GAAGTATCAAAAGCGGGGCTTTGGTCAGGACCTACTATGTGATTTTTTTGAACATGTAAAAATAATTCACCAGGCATTAC
CAATTAAAGGGGTTTATCTTGATGCTGACCCTGCCGCCATTAATTTTTATGCTCGTCTCGGCTTTGTTCAGCTTTCAGCG
ACACCAAATGCTTTTGGTGCTGTACCTATGTTTTTGGCGATTCAGCATATTCTCGCGGCTTAG
Antitoxin
Download Length: 104 a.a. Molecular weight: 11358.04 Da Isoelectric Point: 10.4825
>AT4502 157159952 NC_009800:c512694-512383 [Escherichia coli HS]
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA
MPGKTATLPDVDKTLKNARVELKTSPDAKNKLREAAQAVGVDLSAFILSAAMERAESVLDNQRRRELSNQSWELMNQLIA
EPAQPTLALKALMKRKNSDGRQA
Download Length: 312 bp
>AT4502 NC_009800:c512694-512383 [Escherichia coli HS]
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA
ATGCCTGGAAAAACAGCCACTCTACCCGATGTCGATAAAACGCTGAAAAATGCGCGGGTCGAACTCAAAACTAGTCCCGA
CGCCAAAAACAAACTTCGTGAAGCTGCGCAAGCCGTTGGGGTAGATTTAAGCGCTTTTATTTTGAGCGCTGCTATGGAAC
GCGCCGAAAGTGTGCTTGATAACCAACGTCGCCGTGAGCTTTCGAATCAAAGCTGGGAACTGATGAACCAACTCATCGCT
GAACCTGCTCAACCGACGCTCGCCCTCAAGGCGTTAATGAAAAGGAAAAACAGCGATGGTCGACAAGCATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT10120 | Klebsiella pneumoniae subsp. pneumoniae HS11286 |
35.802 |
92.045 |
0.33 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Brendan Wilcox et al. (2018) Escherichia coli ItaT is a type II toxin that inhibits translation by acetylating isoleucyl-tRNAIle. Nucleic Acids Research 46(15):7873-7885. [PubMed:29931259]