Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ataRT/RHH-GNAT |
| Location | 4335181..4335941 | Replicon | chromosome |
| Accession | NC_002655 | ||
| Organism | Escherichia coli O157:H7 str. EDL933 | ||
Toxin (Protein)
| Gene name | ataT | Uniprot ID | - |
| Locus tag | Z4777 | Protein ID | 15803926 |
| Coordinates | 4335456..4335941 (+) | Length | 162 a.a. |
Antitoxin (Protein)
| Gene name | ataR | Uniprot ID | - |
| Locus tag | Z4776 | Protein ID | 15803925 |
| Coordinates | 4335181..4335465 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| Z_RS22460 (Z4774) | 4332415..4335120 | + | 2706 | WP_000906970.1 | HTH-type transcriptional regulator MalT | - |
| - | 4335181..4335465 | + | 285 | - | - | Antitoxin |
| Z_RS22470 (Z4777) | 4335456..4335941 | + | 486 | WP_001301452.1 | GNAT family N-acetyltransferase | Toxin |
| Z_RS22475 (Z4778) | 4335991..4337019 | - | 1029 | WP_010904929.1 | RNA 3'-terminal phosphate cyclase | - |
| Z_RS22480 (Z4779) | 4337023..4338249 | - | 1227 | WP_001105473.1 | RNA-splicing ligase RtcB | - |
| Z_RS22485 (Z4780) | 4338437..4340035 | + | 1599 | WP_001232986.1 | DNA-binding transcriptional regulator RtcR | - |
| Z_RS22490 (Z4781) | 4340017..4340775 | - | 759 | WP_001302007.1 | DeoR/GlpR family transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 162 a.a. Molecular weight: 17650.57 Da Isoelectric Point: 9.4066
>T199 15803926 NC_002655:4335456-4335941 [Escherichia coli O157:H7 str. EDL933]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E
Download Length: 486 bp
>T199 NC_002655:4335456-4335941 [Escherichia coli O157:H7 str. EDL933]
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA
ATGGGAATAACGGCTCCTACTCCCCTAACGTCTGAACATAATCTGGCTGACTTTTGCTGTTCCGATCACGGGATGAATGA
ATGGCTAAAAAAGAAAGCGTTAAAAAATCACAGCTCCGGTCTGTCTCGTGTTTACGTTATCTGTATTGCCAATACCCGCC
AGGTAATCGGCTATTACTGCCTTTCTACAGGCAGTATTCAACGTAATTTGGCCCCTGGAGCCATGCGACGTAATGCTCCC
GAATCGTTGCCTGTTGTCGTATTAGGCAGACTGGCAATTGATCAAGCCTGGGCAGGTAAAGGGTTGGGCGTCGCATTGTT
GAAAGATGCAGTCTATCGCACAATGTCCATAGCTCAACAGGTAGGGGTTCGTGCGCTCATTGTTCATGCCTTAGACGATT
CAGTACGCAATTTCTATCTGAAGTATGCCTTTGTACCTTCACCATTCCAGTCACTTACCTTACTGTATCCCATTACTCTG
GAGTAA
Antitoxin
Download Length: 95 a.a. Molecular weight: 10666.24 Da Isoelectric Point: 4.6336
>AT199 15803925 NC_002655:4335181-4335465 [Escherichia coli O157:H7 str. EDL933]
MNMKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQ
AKINALMNRKSPWE
MNMKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFILDDEQYDAFLAALDAPITAERQ
AKINALMNRKSPWE
Download Length: 285 bp
>AT199 NC_002655:4335181-4335465 [Escherichia coli O157:H7 str. EDL933]
ATGAATATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGC
AGCGAATCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATC
AACGACTATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAG
GCCAAAATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA
ATGAATATGAAACCGGAAAGCAAAGAAGCGCCAATCAATATCAGAGCGAAAGCCTCACAGCGTGACCTGATTGATATGGC
AGCGAATCTGGTCGCAAAATCGCGCACTGATTTTATGCTGGATGCCGCATGCCGTGAAGCGCAAGACATCTTATTAGATC
AACGACTATTCATCCTTGATGACGAGCAATACGATGCGTTTCTCGCTGCCCTGGACGCCCCCATCACTGCTGAGCGTCAG
GCCAAAATTAACGCGTTGATGAATCGTAAATCGCCATGGGAATAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T443 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
45.098 |
95.031 |
0.429 |
| T10051 | Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023 |
41.83 |
95.031 |
0.398 |
| T10041 | Mycobacterium tuberculosis H37Rv |
39.61 |
95.652 |
0.379 |
| T10052 | Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023 |
35.172 |
90.062 |
0.317 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT443 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
48.193 |
88.298 |
0.426 |
| AT10051 | Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023 |
41.489 |
100 |
0.415 |
| AT10041 | Mycobacterium tuberculosis H37Rv |
40.789 |
80.851 |
0.33 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Stepan V Ovchinnikov et al. (2020) Mechanism of translation inhibition by type II GNAT toxin AtaT2. Nucleic Acids Research 48(15):8617-8625. [PubMed:32597957]