Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | tacAT/- |
| Location | 3092210..3092991 | Replicon | chromosome |
| Accession | UFRD01000002 | ||
| Organism | Salmonella enterica subsp. enterica serovar Typhimurium strain NCTC12023 | ||
Toxin (Protein)
| Gene name | TacT2 | Uniprot ID | - |
| Locus tag | NCTC12023_03333 | Protein ID | SUH98649.1 |
| Coordinates | 3092210..3092701 (-) | Length | 164 a.a. |
Antitoxin (Protein)
| Gene name | TacA2 | Uniprot ID | - |
| Locus tag | NCTC12023_03334 | Protein ID | SUH98650.1 |
| Coordinates | 3092698..3092991 (-) | Length | 98 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| NCTC12023_03328 | 3088205..3088780 | + | 576 | SUH98645.1 | transcriptional regulator | - |
| NCTC12023_03330 | 3089219..3089551 | - | 333 | SUH98646.1 | MerR family transcriptional regulator | - |
| NCTC12023_03331 | 3089623..3090000 | + | 378 | SUH98647.1 | ethyl tert-butyl ether degradation EthD | - |
| NCTC12023_03332 | 3091210..3091962 | + | 753 | SUH98648.1 | acid phosphatase | - |
| NCTC12023_03333 | 3092210..3092701 | - | 492 | SUH98649.1 | N-acetyltransferase GCN5 | Toxin |
| NCTC12023_03334 | 3092698..3092991 | - | 294 | SUH98650.1 | Uncharacterized protein conserved in bacteria | Antitoxin |
| NCTC12023_03335 | 3093793..3094668 | + | 876 | SUH98651.1 | AraC family transcriptional regulator | - |
| NCTC12023_03336 | 3094680..3094904 | + | 225 | SUH98652.1 | GerE family regulatory protein | - |
| NCTC12023_03337 | 3095104..3095466 | + | 363 | SUH98653.1 | putative phage protein | - |
| NCTC12023_03339 | 3095760..3096620 | - | 861 | SUH98654.1 | membrane protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 164 a.a. Molecular weight: 17646.39 Da Isoelectric Point: 6.8437
>T10051 SUH98649.1 UFRD01000002:c3092701-3092210 [Salmonella enterica subsp. enterica serovar Typhimurium]
MISTPEPLHAGHILTPFCCGVDSIDNWLEQRAMKNQTTGASRTFVCCGSDSNVLAYYSLASSAVTTNTSPGRFRRNMPDP
IPVVVLGRLAVDKSLHGQGVARALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYQRVGFVPSPMDPMMLMVTLGDLV
ESV
MISTPEPLHAGHILTPFCCGVDSIDNWLEQRAMKNQTTGASRTFVCCGSDSNVLAYYSLASSAVTTNTSPGRFRRNMPDP
IPVVVLGRLAVDKSLHGQGVARALVRDAGLRVIQVAETIGIRGMLVHALSDEAREFYQRVGFVPSPMDPMMLMVTLGDLV
ESV
Download Length: 492 bp
>T10051 UFRD01000002:c3092701-3092210 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA
ATGATCTCCACCCCTGAGCCGCTTCATGCCGGACATATTCTTACTCCGTTTTGCTGCGGTGTGGATTCTATAGATAACTG
GCTGGAACAGCGGGCGATGAAAAATCAGACCACTGGCGCGTCCCGTACCTTTGTGTGTTGTGGCAGCGATTCGAACGTAC
TGGCCTATTACTCGCTGGCGTCCAGCGCGGTCACGACGAATACCTCCCCCGGTCGCTTTCGTCGCAATATGCCTGACCCG
ATTCCGGTTGTGGTATTGGGGCGTCTGGCGGTGGATAAATCGCTTCATGGGCAGGGCGTCGCTCGGGCGCTGGTACGCGA
CGCAGGGCTGCGGGTTATTCAGGTGGCGGAGACTATCGGCATTCGTGGGATGCTGGTTCACGCCCTGTCGGATGAAGCGC
GGGAATTTTATCAGCGGGTGGGGTTTGTACCGTCGCCGATGGATCCGATGATGTTGATGGTGACGTTGGGGGATTTGGTG
GAGAGTGTTTAA
Antitoxin
Download Length: 98 a.a. Molecular weight: 10977.61 Da Isoelectric Point: 8.6141
>AT10051 SUH98650.1 UFRD01000002:c3092991-3092698 [Salmonella enterica subsp. enterica serovar Typhimurium]
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLVRLDQTPSPN
AALRKTMQTPAPWEQEK
MPAANSMAMKRETLNLRIKPAERDLIDRAAKARGKNRTDFVLEAARAAAEEALIEQRIIMADPEAYQEFLVRLDQTPSPN
AALRKTMQTPAPWEQEK
Download Length: 294 bp
>AT10051 UFRD01000002:c3092991-3092698 [Salmonella enterica subsp. enterica serovar Typhimurium]
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA
ATGCCAGCCGCAAACAGTATGGCGATGAAACGCGAAACCCTGAACCTGCGTATCAAACCCGCCGAGCGCGATCTTATCGA
TCGAGCGGCGAAAGCCAGAGGGAAAAACCGCACAGACTTTGTGCTGGAAGCTGCCCGCGCTGCCGCAGAGGAAGCGCTTA
TCGAACAACGCATCATTATGGCCGATCCAGAGGCTTATCAGGAGTTTCTCGTTCGTTTGGATCAAACACCTTCACCTAAT
GCCGCACTGCGTAAAACCATGCAGACCCCTGCGCCGTGGGAGCAGGAAAAATGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| T443 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
47.436 |
96.894 |
0.46 |
| T10041 | Mycobacterium tuberculosis H37Rv |
46.795 |
95.706 |
0.448 |
| T199 | Escherichia coli O157:H7 str. EDL933 |
41.83 |
95.031 |
0.398 |
Multiple sequence alignment
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| AT199 | Escherichia coli O157:H7 str. EDL933 |
41.489 |
100 |
0.415 |
| AT443 | Salmonella enterica subsp. enterica serovar Typhimurium str. LT2 |
38.272 |
85.263 |
0.326 |
Multiple sequence alignment
Structures
Toxin
| Source | ID | Structure |
|---|
Antitoxin
| Source | ID | Structure |
|---|
References
(1) Julian A Rycroft et al. (2018) Activity of acetyltransferase toxins involved in Salmonella persister formation during macrophage infection. Nature Communications 9(1):1993. [PubMed:29777131]
(2) Grzegorz J Grabe et al. (2021) Auxiliary interfaces support the evolution of specific toxin-antitoxin pairing. Nature Chemical Biology 17(12):1296-1304. [PubMed:34556858]