Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/-
Location 796990..797598 Replicon chromosome
Accession NC_002516
Organism Pseudomonas aeruginosa PAO1

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag PA0729 Protein ID NP_249420.1
Coordinates 797251..797598 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID -
Locus tag - Protein ID -
Coordinates 796990..797241 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
PA0725 792637..792993 + 357 NP_249416.1 hypothetical protein -
PA0726 792997..794271 + 1275 NP_249417.1 hypothetical protein -
PA0727 794501..795793 + 1293 NP_249418.1 hypothetical protein -
PA0728 795793..796776 + 984 NP_249419.1 bacteriophage integrase -
- 796990..797241 + 252 - - Antitoxin
PA0729 797251..797598 + 348 NP_249420.1 hypothetical protein Toxin
PA0730 797925..798827 - 903 NP_249421.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
PA0731 799279..800016 - 738 NP_249422.1 hypothetical protein -
PA0732 800096..801139 - 1044 NP_249423.1 hypothetical protein -
PA0733 801275..801967 - 693 NP_249424.1 16S rRNA pseudouridine(516) synthase -
PA0734 801967..802239 - 273 NP_249425.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 783009..797598 14589


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T10100 NP_249420.1 NC_002516:797251-797598 [Pseudomonas aeruginosa PAO1]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T10100 NC_002516:797251-797598 [Pseudomonas aeruginosa PAO1]
ATGTCCCCGGTCGTCATTCGTTTTACTGATACCGCAGAGCAAAGCATCGAAGACCAAGTCCACCACTTGGCTCCATTCCA
AGGTGAACAGGCTGCACTCCAGTCAGTACTGAGCCTTTTGGATGAGATTGAAGAGAAGATTTCACTTGCACCTAAAGGTT
ACCCAGTCAGCCAGCAGGCGAGTCTTCTGGGGGTGCTGAGCTATCGCGAGCTTAATACCGGCCCCTATCGTGTTTTTTAC
GAATTCCACGAAGAGCAAGGCGAGGTGGCAGTGATCTTGGTTTTGCGACAGAAGCAGAGCGTTGAGCAGCAATTGATCCG
CTACTGCTTGGTGGGGCCAATCGAGTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT10100 - NC_002516:796990-797241 [Pseudomonas aeruginosa PAO1]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT10100 NC_002516:796990-797241 [Pseudomonas aeruginosa PAO1]
ATGCGAGTCGAGACAATTAGTTATTTGAAACGTCATGCGGCTGACCTGGATTTATCCGAGCCAATGGTCGTCACGCAGAA
CGGTGTTCCTGCCTATGTGGTTGAGTCATATGCTGAGCGGAAGCAGCGCGATGAAGCAATTGCGCTGGTGAAGTTGCTTG
CGATTGGCTCCCGCCAGTACGCAGAAGGCAAGCATCGCTCTGTTGATGATTTGAAAGCTCGCCTTTCCAGGAGGTTCGCT
CAGCCAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value
AT178 Vibrio cholerae O1 biovar El Tor str. N16961

38.824

100

0.398

Multiple sequence alignment    




Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure

References

(1) Itzhak Zander et al. (2020) Characterization of PfiT/PfiA toxin-antitoxin system of Pseudomonas aeruginosa that affects cell elongation and prophage induction. Environmental Microbiology 22(12):5048-5057. [PubMed:32458560] experimental

(2) Yangmei Li et al. (2020) Prophage encoding toxin/antitoxin system PfiT/PfiA inhibits Pf4 production in Pseudomonas aeruginosa. Microbial Biotechnology 13(4):1132-1144. [PubMed:32246813] experimental


experimental experimental literature