220410

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) PfiT-PfiA/ParE(toxin)
Location 796772..797380 Replicon chromosome
Accession NZ_CP085082
Organism Pseudomonas aeruginosa strain PAO1-Holloway

Toxin (Protein)


Gene name PfiT Uniprot ID Q9I5J9
Locus tag LIO20_RS03750 Protein ID WP_003114156.1
Coordinates 797033..797380 (+) Length 116 a.a.

Antitoxin (Protein)


Gene name PfiA Uniprot ID A0A0B0C355
Locus tag LIO20_RS03745 Protein ID WP_003114155.1
Coordinates 796772..797023 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LIO20_RS03725 (LIO20_03720) 792419..792775 + 357 WP_003114150.1 DUF2523 family protein -
LIO20_RS03730 (LIO20_03725) 792779..794053 + 1275 WP_010895520.1 zonular occludens toxin family protein -
LIO20_RS03735 (LIO20_03730) 794283..795575 + 1293 WP_003115206.1 hypothetical protein -
LIO20_RS03740 (LIO20_03735) 795575..796558 + 984 WP_003114154.1 tyrosine-type recombinase/integrase -
LIO20_RS03745 (LIO20_03740) 796772..797023 + 252 WP_003114155.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LIO20_RS03750 (LIO20_03745) 797033..797380 + 348 WP_003114156.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LIO20_RS03760 (LIO20_03755) 797707..798609 - 903 WP_003114157.1 (R)-3-hydroxydecanoyl-ACP:CoA transacylase -
LIO20_RS03765 (LIO20_03760) 799061..799798 - 738 WP_003114158.1 murein L,D-transpeptidase catalytic domain family protein -
LIO20_RS03770 (LIO20_03765) 799878..800921 - 1044 WP_003098363.1 L,D-transpeptidase -
LIO20_RS03775 (LIO20_03770) 801057..801749 - 693 WP_003114159.1 16S rRNA pseudouridine(516) synthase -
LIO20_RS03780 (LIO20_03775) 801749..802021 - 273 WP_010895521.1 cysteine-rich CWC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Prophage - - 782014..797380 15366


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-100)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 116 a.a.        Molecular weight: 12984.78 Da        Isoelectric Point: 4.4212

>T220410 WP_003114156.1 NZ_CP085082:797033-797380 [Pseudomonas aeruginosa]
MSPVVIRFTDTAEQSIEDQVHHLAPFQGEQAALQSVLSLLDEIEEKISLAPKGYPVSQQASLLGVLSYRELNTGPYRVFY
EFHEEQGEVAVILVLRQKQSVEQQLIRYCLVGPIE

Download         Length: 348 bp

>T220410 NZ_CP117016:3615741-3616115 [Escherichia coli]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGGTTACTGGACCAGCATTACAGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGCGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCAGTGAACTTTCTCGTGGAAAAATACGCACTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGCCTCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCCA
CAACTACAGAACGGTGAACAATATCACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9442.79 Da        Isoelectric Point: 9.4808

>AT220410 WP_003114155.1 NZ_CP085082:796772-797023 [Pseudomonas aeruginosa]
MRVETISYLKRHAADLDLSEPMVVTQNGVPAYVVESYAERKQRDEAIALVKLLAIGSRQYAEGKHRSVDDLKARLSRRFA
QPE

Download         Length: 252 bp

>AT220410 NZ_CP117016:3615283-3615651 [Escherichia coli]
GTGTCAGACACACTTTCCGGGACAACGCATCCCGATGACAATGACGACCATCCCTGGTGGGGACTACCCTGCACAGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGT
TCAGAGATGCGGATGCGTACCCTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGT
GAACTGAATCCCCGCCATCAGCATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCTGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB Q9I5J9

Antitoxin

Source ID Structure
AlphaFold DB A0A0B0C355

References