68442

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3169001..3169800 Replicon chromosome
Accession NZ_CP017979
Organism Escherichia coli str. K-12 substr. W3110 strain K-12 substr. ZK126

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag BL257_RS16795 Protein ID WP_000347273.1
Coordinates 3169336..3169800 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag BL257_RS16790 Protein ID WP_001307405.1
Coordinates 3169001..3169336 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BL257_RS16775 (BL257_15720) 3164786..3165556 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
BL257_RS16780 (BL257_15725) 3165572..3166906 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
BL257_RS16785 (BL257_15730) 3167281..3168852 + 1572 WP_001273753.1 galactarate dehydratase -
BL257_RS16790 (BL257_15735) 3169001..3169336 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
BL257_RS16795 (BL257_15740) 3169336..3169800 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
BL257_RS16800 (BL257_15745) 3169855..3170664 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
BL257_RS16810 (BL257_15750) 3170913..3172193 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
BL257_RS16815 (BL257_15755) 3172216..3172689 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
BL257_RS16820 (BL257_15760) 3172700..3173071 + 372 Protein_3033 PTS sugar transporter subunit IIC -
BL257_RS16825 (BL257_15765) 3173067..3173624 + 558 Protein_3034 amidohydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3158126..3169800 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T68442 WP_000347273.1 NZ_CP017979:3169336-3169800 [Escherichia coli str. K-12 substr. W3110]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T68442 NZ_CP017979:3169336-3169800 [Escherichia coli str. K-12 substr. W3110]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT68442 WP_001307405.1 NZ_CP017979:3169001-3169336 [Escherichia coli str. K-12 substr. W3110]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT68442 NZ_CP017979:3169001-3169336 [Escherichia coli str. K-12 substr. W3110]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References