Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1725892..1726691 Replicon chromosome
Accession NZ_CP063511
Organism Escherichia coli strain EH08-18-36

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag IQS18_RS08340 Protein ID WP_000347273.1
Coordinates 1725892..1726356 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID V0TI39
Locus tag IQS18_RS08345 Protein ID WP_021547164.1
Coordinates 1726356..1726691 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
IQS18_RS08310 1720893..1721327 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
IQS18_RS08315 1721345..1722223 - 879 WP_001300474.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
IQS18_RS08320 1722213..1722992 - 780 WP_203024061.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
IQS18_RS08325 1723003..1723476 - 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
IQS18_RS08330 1723499..1724779 - 1281 WP_000681903.1 tagatose-bisphosphate aldolase subunit KbaZ -
IQS18_RS08335 1725028..1725837 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
IQS18_RS08340 1725892..1726356 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
IQS18_RS08345 1726356..1726691 - 336 WP_021547164.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
IQS18_RS08350 1726840..1728411 - 1572 WP_203024062.1 galactarate dehydratase -
IQS18_RS08355 1728786..1730120 + 1335 WP_000599651.1 galactarate/glucarate/glycerate transporter GarP -
IQS18_RS08360 1730136..1730906 + 771 WP_053270423.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1725892..1737566 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T178577 WP_000347273.1 NZ_CP063511:c1726356-1725892 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T178577 NZ_CP084109:c188800-188540 [Micrococcus luteus]
ATGTCCATCAGTGCCAGTGAAGCCCGCAAGACCCTGTTCCCGCTCATCGAGCGAGTGAATGCGGACCGCGAGGTCGTGGA
GATCGTCTCCCGCAAGGGGAATGCCGTCCTCATGCCCGCGGACGAGTACGCCGCCTGGCAGGAGACCGCCTACCTGTTCC
GCTCCCCGGCCAACGCCCGCCGGCTGCTGGACGCCTACGAGCGCGCCCGCGCAGGGAGCGTCGAGGAGCACGCTCTGGAC
CGCATGGACGGGGACGCCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12309.86 Da        Isoelectric Point: 4.5392

>AT178577 WP_021547164.1 NZ_CP063511:c1726691-1726356 [Escherichia coli]
MPANARSNAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT178577 NZ_CP084109:c188539-188285 [Micrococcus luteus]
GTGCGCCTCGTGTGGGACCGCTCGGCCTGGGAGGACTACACGCACTGGCAGACCACGGACCGCAAGGTCCTCAAGCGGAT
CAACACCCTCATCGACGCCGCCCTGCGTGATCCCTTCGACGGGATCGGCAAGCCCGAGCAGCTGAAGTACGGCGCGCCCG
GGGCATGGTCCCGACGCATCACGGAGGAGCACAGGCTCGTCTACCTCGTCGACGGCGAGGACCTGATCATCCTCCAGGTG
AGGTATCACTACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB V0TI39

References