285025

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3504819..3505618 Replicon chromosome
Accession NZ_HG738867
Organism Escherichia coli str. K-12 substr. MC4100 strain K-12

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag BN896_RS17360 Protein ID WP_000347273.1
Coordinates 3504819..3505283 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag BN896_RS17365 Protein ID WP_001307405.1
Coordinates 3505283..3505618 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BN896_RS17335 3500995..3501552 - 558 Protein_3285 amidohydrolase family protein -
BN896_RS17340 3501548..3501919 - 372 Protein_3286 PTS sugar transporter subunit IIC -
BN896_RS17345 (BN896_2839) 3501930..3502403 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
BN896_RS17350 (BN896_2838) 3502426..3503706 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
BN896_RS17355 (BN896_2837) 3503955..3504764 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
BN896_RS17360 (BN896_2836) 3504819..3505283 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
BN896_RS17365 (BN896_2835) 3505283..3505618 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
BN896_RS17370 (BN896_2834) 3505767..3507338 - 1572 WP_001273753.1 galactarate dehydratase -
BN896_RS17375 (BN896_2833) 3507713..3509047 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
BN896_RS17380 (BN896_2832) 3509063..3509833 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3504819..3516493 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T285025 WP_000347273.1 NZ_HG738867:c3505283-3504819 [Escherichia coli str. K-12 substr. MC4100]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T285025 NZ_HG738867:c3505283-3504819 [Escherichia coli str. K-12 substr. MC4100]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT285025 WP_001307405.1 NZ_HG738867:c3505618-3505283 [Escherichia coli str. K-12 substr. MC4100]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT285025 NZ_HG738867:c3505618-3505283 [Escherichia coli str. K-12 substr. MC4100]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References