256472

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 608325..609124 Replicon chromosome
Accession NZ_CP103745
Organism Escherichia coli strain 3090

Toxin (Protein)


Gene name yhaV Uniprot ID A0A0A6SPA6
Locus tag M5T02_RS02970 Protein ID WP_000347275.1
Coordinates 608325..608789 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag M5T02_RS02975 Protein ID WP_001307405.1
Coordinates 608789..609124 (-) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
M5T02_RS02940 (603326) 603326..603760 - 435 WP_000948824.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -
M5T02_RS02945 (603778) 603778..604656 - 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
M5T02_RS02950 (604646) 604646..605425 - 780 WP_000406209.1 PTS N-acetylgalactosamine transporter subunit IIC -
M5T02_RS02955 (605436) 605436..605909 - 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
M5T02_RS02960 (605932) 605932..607212 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
M5T02_RS02965 (607461) 607461..608270 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
M5T02_RS02970 (608325) 608325..608789 - 465 WP_000347275.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
M5T02_RS02975 (608789) 608789..609124 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
M5T02_RS02980 (609273) 609273..610844 - 1572 WP_259429197.1 galactarate dehydratase -
M5T02_RS02985 (611219) 611219..612553 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
M5T02_RS02990 (612569) 612569..613339 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 608325..619999 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17820.29 Da        Isoelectric Point: 9.8492

>T256472 WP_000347275.1 NZ_CP103745:c608789-608325 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKVPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT256472 WP_001307405.1 NZ_CP103745:c609124-608789 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp


Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0A6SPA6

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References