22703

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3372769..3373568 Replicon chromosome
Accession NC_010473
Organism Escherichia coli str. K-12 substr. DH10B

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag ECDH10B_RS16785 Protein ID WP_000347273.1
Coordinates 3373104..3373568 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag ECDH10B_RS16780 Protein ID WP_001307405.1
Coordinates 3372769..3373104 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECDH10B_RS16765 (ECDH10B_3299) 3368554..3369324 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
ECDH10B_RS16770 (ECDH10B_3300) 3369340..3370674 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
ECDH10B_RS16775 (ECDH10B_3301) 3371049..3372620 + 1572 WP_001273753.1 galactarate dehydratase -
ECDH10B_RS16780 (ECDH10B_3302) 3372769..3373104 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
ECDH10B_RS16785 (ECDH10B_3303) 3373104..3373568 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
ECDH10B_RS16790 (ECDH10B_3304) 3373623..3374432 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
ECDH10B_RS16795 (ECDH10B_3305) 3374681..3375961 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
ECDH10B_RS16800 (ECDH10B_3306) 3375984..3376457 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
ECDH10B_RS16805 (ECDH10B_3307) 3376468..3376839 + 372 Protein_3208 PTS sugar transporter subunit IIC -
ECDH10B_RS16810 (ECDH10B_3308) 3376835..3377392 + 558 Protein_3209 amidohydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3363621..3373568 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T22703 WP_000347273.1 NC_010473:3373104-3373568 [Escherichia coli str. K-12 substr. DH10B]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T22703 NC_010473:3373104-3373568 [Escherichia coli str. K-12 substr. DH10B]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT22703 WP_001307405.1 NC_010473:3372769-3373104 [Escherichia coli str. K-12 substr. DH10B]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT22703 NC_010473:3372769-3373104 [Escherichia coli str. K-12 substr. DH10B]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References