187075

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 4523860..4524659 Replicon chromosome
Accession NZ_CP068281
Organism Escherichia coli strain CY706

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag JJW04_RS22015 Protein ID WP_000347273.1
Coordinates 4524195..4524659 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag JJW04_RS22010 Protein ID WP_001307405.1
Coordinates 4523860..4524195 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
JJW04_RS21995 (4519645) 4519645..4520415 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
JJW04_RS22000 (4520431) 4520431..4521765 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
JJW04_RS22005 (4522140) 4522140..4523711 + 1572 WP_001273753.1 galactarate dehydratase -
JJW04_RS22010 (4523860) 4523860..4524195 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
JJW04_RS22015 (4524195) 4524195..4524659 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
JJW04_RS22020 (4524714) 4524714..4525523 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
JJW04_RS22025 (4525772) 4525772..4527052 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
JJW04_RS22030 (4527075) 4527075..4527548 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
JJW04_RS22035 (4527559) 4527559..4527930 + 372 Protein_4305 PTS sugar transporter subunit IIC -
JJW04_RS22040 (4527926) 4527926..4528483 + 558 Protein_4306 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 4514712..4524659 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T187075 WP_000347273.1 NZ_CP068281:4524195-4524659 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T187075 NZ_CP089777:1971739-1971987 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT187075 WP_001307405.1 NZ_CP068281:4523860-4524195 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT187075 NZ_CP089777:1971527-1971739 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References