1047

Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/AbrB-RelE
Location 3275024..3275823 Replicon chromosome
Accession NC_000913
Organism Escherichia coli str. K-12 substr. MG1655

Toxin (Protein)


Gene name yhaV Uniprot ID P64594
Locus tag b3130 Protein ID NP_417599.1
Coordinates 3277337..3277801 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID P15373
Locus tag b3129 Protein ID NP_417598.1
Coordinates 3277002..3277337 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
b3124 3270625..3271770 - 1146 NP_417593.4 glycerate 2-kinase 1 -
b3125 3271867..3272751 - 885 NP_417594.3 tartronate semialdehyde reductase -
b3126 3272787..3273557 - 771 NP_417595.1 alpha-dehydro-beta-deoxy-D-glucarate aldolase -
b3127 3273573..3274907 - 1335 NP_417596.1 galactarate/D-glucarate transporter GarP -
b3128 3275282..3276853 + 1572 NP_417597.1 GarD -
b3129 3277002..3277337 + 336 NP_417598.1 antitoxin PrlF Antitoxin
b3130 3277337..3277801 + 465 NP_417599.1 ribosome-dependent mRNA interferase toxin YhaV Toxin
b3131 3277856..3278665 - 810 NP_417600.1 DNA-binding transcriptional repressor AgaR -
b3132 3278914..3280194 + 1281 NP_417601.1 putative tagatose-1,6-bisphosphate aldolase 1 chaperone -
b3133 3280217..3280690 + 474 NP_417602.4 N-acetyl-D-galactosamine specific PTS enzyme IIB component -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3267854..3277801 9947


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T1047 NP_417599.1 NC_000913:3277337-3277801 [Escherichia coli str. K-12 substr. MG1655]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T1047 NC_000913:3277337-3277801 [Escherichia coli str. K-12 substr. MG1655]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT1047 NP_417598.1 NC_000913:3277002-3277337 [Escherichia coli str. K-12 substr. MG1655]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT1047 NC_000913:3277002-3277337 [Escherichia coli str. K-12 substr. MG1655]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P64594


Antitoxin

Source ID Structure
AlphaFold DB P15373

References


(1) J Paul Norton et al. (2012) Toxin-antitoxin systems are important for niche-specific colonization and stress resistance of uropathogenic Escherichia coli. PLoS Pathogens 8(10):e1002954. [PubMed:23055930] experimental

(2) Wonho Choi et al. (2018) Functional Characterization of the C-Terminus of YhaV in the Escherichia coli PrlF-YhaV Toxin-Antitoxin System. Journal of Microbiology And Biotechnology 28(6):987-996. [PubMed:29642286] experimental


experimental experimental literature