218795

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 1266975..1267774 Replicon chromosome
Accession NZ_CP084529
Organism Escherichia coli strain Origami2-DE3

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag LFW28_RS06125 Protein ID WP_000347273.1
Coordinates 1267310..1267774 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag LFW28_RS06120 Protein ID WP_001307405.1
Coordinates 1266975..1267310 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LFW28_RS06105 (1262760) 1262760..1263530 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
LFW28_RS06110 (1263546) 1263546..1264880 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
LFW28_RS06115 (1265255) 1265255..1266826 + 1572 WP_001273753.1 galactarate dehydratase -
LFW28_RS06120 (1266975) 1266975..1267310 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
LFW28_RS06125 (1267310) 1267310..1267774 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
LFW28_RS06130 (1267829) 1267829..1268638 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
LFW28_RS06135 (1268887) 1268887..1270167 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
LFW28_RS06140 (1270190) 1270190..1270663 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
LFW28_RS06145 (1270674) 1270674..1271045 + 372 Protein_1208 PTS sugar transporter subunit IIC -
LFW28_RS06150 (1271041) 1271041..1271598 + 558 Protein_1209 amidohydrolase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 1257262..1267774 10512


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T218795 WP_000347273.1 NZ_CP084529:1267310-1267774 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T218795 NZ_CP116101:139758-140174 [Escherichia coli]
GTGAACAAGATCTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAACCTGAAGCATTGCTTAAGCACCT
GGAGCAGTCGGTGCTGCGCGGTCACCGTATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGTGCCACCGGTC
CGAAGGCCTCGCCACGCCACGTGCAACTGGTTGACGCGTTCTGTGAGAGGCTCGATGCCGTCCTGCCGTGGGACCGCGCC
GCGGTGGATGCGACCACGGAGATTAAGGTGGCACTGCGTCTCGCCGGCACGCCGATCGGTCCGAACGACACGGCGATAGC
CGGACACGCCATCGCTGCCTGTGCCATTCTGGTGACGAATAATGTGAGAGAGTTTGAGCGGGTACCGGGTCTTGTGCTGG
AAGACTGGGTAAGATAA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT218795 WP_001307405.1 NZ_CP084529:1266975-1267310 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT218795 NZ_CP116101:139531-139761 [Escherichia coli]
ATGAGAACCGTATCCATTTTTAAAAATGGCAACAACCGTGCTATCCGCCTTCCACGCGATCTGGATTTTGAGGGGGTGAG
CGAGCTGGAGATCGTCCGGGAAGGGGACAGCATCATTCTGCGCCCCGTCCGGCCGACCTGGGGCTCATTCCTGGAGCTAG
AAAAGGCCGATCCGGACTTTATGACGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGAGTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SSH7

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References