75057

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 640607..641406 Replicon chromosome
Accession NZ_CP020543
Organism Escherichia coli C

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag B6N50_RS03280 Protein ID WP_000347273.1
Coordinates 640607..641071 (-) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag B6N50_RS03285 Protein ID WP_001307405.1
Coordinates 641071..641406 (-) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
B6N50_RS03245 (B6N50_03245) 635608..636042 - 435 WP_000948824.1 PTS sugar transporter subunit IIA -
B6N50_RS03250 (B6N50_03250) 636060..636938 - 879 WP_001295548.1 PTS system mannose/fructose/sorbose family transporter subunit IID -
B6N50_RS03255 (B6N50_03255) 636928..637707 - 780 WP_000406209.1 PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC -
B6N50_RS03260 (B6N50_03260) 637718..638191 - 474 WP_081508768.1 PTS N-acetylgalactosamine transporter subunit IIB -
B6N50_RS03265 (B6N50_03265) 638214..639494 - 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
B6N50_RS03275 (B6N50_03275) 639743..640552 + 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
B6N50_RS03280 (B6N50_03280) 640607..641071 - 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
B6N50_RS03285 (B6N50_03285) 641071..641406 - 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
B6N50_RS03290 (B6N50_03290) 641555..643126 - 1572 WP_001273753.1 galactarate dehydratase -
B6N50_RS03295 (B6N50_03295) 643501..644835 + 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
B6N50_RS03300 (B6N50_03300) 644851..645621 + 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 640607..652281 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T75057 WP_000347273.1 NZ_CP020543:c641071-640607 [Escherichia coli C]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T75057 NZ_CP020543:c641071-640607 [Escherichia coli C]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT75057 WP_001307405.1 NZ_CP020543:c641406-641071 [Escherichia coli C]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT75057 NZ_CP020543:c641406-641071 [Escherichia coli C]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References