118840

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 2934459..2935258 Replicon chromosome
Accession NZ_CP035855
Organism Escherichia coli TW11681

Toxin (Protein)


Gene name yhaV Uniprot ID S1NYM6
Locus tag EXE31_RS14310 Protein ID WP_000347251.1
Coordinates 2934794..2935258 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag EXE31_RS14305 Protein ID WP_001307405.1
Coordinates 2934459..2934794 (+) Length 112 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EXE31_RS14290 (2930244) 2930244..2931014 - 771 WP_001058227.1 2-dehydro-3-deoxyglucarate aldolase -
EXE31_RS14295 (2931030) 2931030..2932364 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
EXE31_RS14300 (2932739) 2932739..2934310 + 1572 WP_001273763.1 galactarate dehydratase -
EXE31_RS14305 (2934459) 2934459..2934794 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
EXE31_RS14310 (2934794) 2934794..2935258 + 465 WP_000347251.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
EXE31_RS14315 (2935313) 2935313..2936122 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
EXE31_RS14320 (2936371) 2936371..2937651 + 1281 WP_000681906.1 tagatose-bisphosphate aldolase subunit KbaZ -
EXE31_RS14325 (2937674) 2937674..2938147 + 474 WP_001295547.1 PTS N-acetylgalactosamine transporter subunit IIB -
EXE31_RS14330 (2938158) 2938158..2938937 + 780 WP_000406213.1 PTS N-acetylgalactosamine transporter subunit IIC -
EXE31_RS14335 (2938927) 2938927..2939805 + 879 WP_001295548.1 PTS N-acetylgalactosamine transporter subunit IID -
EXE31_RS14340 (2939823) 2939823..2940257 + 435 WP_000948827.1 PTS galactosamine/N-acetylgalactosamine transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 2923689..2935258 11569


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin endonuclease, YhaV

(12-150)

Antitoxin

PrlF antitoxin

(14-110)


Sequences

Toxin        


Download         Length: 155 a.a.        Molecular weight: 17777.14 Da        Isoelectric Point: 9.4947

>T118840 WP_000347251.1 NZ_CP035855:2934794-2935258 [Escherichia coli TW11681]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Download         Length: 465 bp

>T118840 NZ_CP035855:2934794-2935258 [Escherichia coli TW11681]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT118840 WP_001307405.1 NZ_CP035855:2934459-2934794 [Escherichia coli TW11681]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT118840 NZ_CP035855:2934459-2934794 [Escherichia coli TW11681]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CJ20

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References