21455

Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) prlF-yhaV (relBE)/YhaV-PrlF
Location 3276857..3277656 Replicon chromosome
Accession NC_007779
Organism Escherichia coli str. K-12 substr. W3110

Toxin (Protein)


Gene name yhaV Uniprot ID V0SSH7
Locus tag Y75_RS16280 Protein ID WP_000347273.1
Coordinates 3277192..3277656 (+) Length 155 a.a.

Antitoxin (Protein)


Gene name prlF Uniprot ID S1EB98
Locus tag Y75_RS16275 Protein ID WP_001307405.1
Coordinates 3276857..3277192 (+) Length 112 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Y75_RS16260 (Y75_p3048) 3272642..3273412 - 771 WP_001058209.1 2-dehydro-3-deoxyglucarate aldolase -
Y75_RS16265 (Y75_p3049) 3273428..3274762 - 1335 WP_000599636.1 galactarate/glucarate/glycerate transporter GarP -
Y75_RS16270 (Y75_p3050) 3275137..3276708 + 1572 WP_001273753.1 galactarate dehydratase -
Y75_RS16275 (Y75_p3051) 3276857..3277192 + 336 WP_001307405.1 type II toxin-antitoxin system antitoxin PrlF Antitoxin
Y75_RS16280 (Y75_p3052) 3277192..3277656 + 465 WP_000347273.1 type II toxin-antitoxin system ribonuclease toxin YhaV Toxin
Y75_RS16285 (Y75_p3053) 3277711..3278520 - 810 WP_000072187.1 aga operon transcriptional regulator AgaR -
Y75_RS16290 (Y75_p3054) 3278769..3280049 + 1281 WP_000681920.1 tagatose-bisphosphate aldolase subunit KbaZ -
Y75_RS16295 (Y75_p3055) 3280072..3280545 + 474 WP_001336162.1 PTS N-acetylgalactosamine transporter subunit IIB -
Y75_RS16300 (Y75_p3056) 3280556..3280927 + 372 Protein_3107 PTS sugar transporter subunit IIC -
Y75_RS16305 (Y75_p3057) 3280923..3281480 + 558 Protein_3108 amidohydrolase family protein -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
inside Genomic island - - 3265982..3277656 11674


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(12-150)

Antitoxin

(14-110)


Sequences


Toxin        


Download         Length: 155 a.a.        Molecular weight: 17836.25 Da        Isoelectric Point: 9.6924

>T21455 WP_000347273.1 NC_007779:3277192-3277656 [Escherichia coli str. K-12 substr. W3110]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Download         Length: 465 bp

>T21455 NC_007779:3277192-3277656 [Escherichia coli str. K-12 substr. W3110]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin


Download         Length: 112 a.a.        Molecular weight: 12358.94 Da        Isoelectric Point: 4.8616

>AT21455 WP_001307405.1 NC_007779:3276857-3277192 [Escherichia coli str. K-12 substr. W3110]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Download         Length: 336 bp

>AT21455 NC_007779:3276857-3277192 [Escherichia coli str. K-12 substr. W3110]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB V0SSH7


Antitoxin

Source ID Structure
AlphaFold DB A0A0E0XVC7

References